摘要
采用酶标ＥＬＩＳＡ夹心法，建立了一种简便、灵敏、特异的检测标本中ＩＬ－８含量的方法，其检测下限达１３．７ｐｇ／ｍｌ。运用此法检测了２０例正常人血浆标本、３０例白血病病人血浆标本和１１例白血病病人脑脊液标本，发现白血病病人标本中ＩＬ－８含量明显高于正常人标本（Ｐ＜０．０５），为临床和科研上检测标本中ＩＬ－８含量提供了一个手段。
y using Sandwich-ELISA, we establiashed a sensitive, samples. This assay could detect the lowest level of IL-8 at a concentration of 13.7μg/L. We applied this assay to various blood plasma samples, including 20 normal human samples, 30 samples from patients with acute Leukemia and 11ones of Celebrale System Fluid (CSF) from patients with acute Leukemia. the results showed thatIL-8 was less than 40μg/L in normal human plasma (32.8±5.1μg/L). However, the IL-8 levelwas significently increased to 72.3±29.6μg/L for acute Lyphmphoblastoid Leukemia (ALL, n=20),99.7±26.5μg/L for Acute Myeloid Leukemia (AML, n=5) and 105.6±34.8μg/L for MultipleMyeloma (MM, n=5). Interestingly, IL-8 level was also higher in CSEs from Leukemia Patientsand reached 108.2±96. 2μg/L (n=11), suggesting that IL-8 might be an important indicator fordiagnosis of celebrale infection and Leukemia invasion as well.
出处
《苏州医学院学报》
1996年第4期778-780,共3页
Acta Academiae Medicinae Suzhou
