摘要
目的研究人类白细胞抗原DRB1(HLA-DRB1)基因多态性与三氯乙烯药疹样皮炎易感性的关系。方法应用聚合酶链反应产物直接测序方法,比较112例三氯乙烯药疹样皮炎病例和142例健康三氯乙烯接触工人HLA-DRB1基因第2外显子氨基酸密码子多态性及等位基因频率分布,并计算相对危险度(OR)及其95%可信区间(95%CI)。结果病例组与接触对照组HLA-DRB1等位基因频率差异有显著性,病例组DRB1*1501、*1202和*04等位基因频率显著高于对照组(分别为18.8%vs7.7%,OR=2.748,95%CI=1.587～4.761;12.5%vs6.0%,OR=2.244,95%CI=1.195～4.214;17.4%vs11.3%,OR=1.660,95%CI=1.002～2.750),而DRB1*0301、*0901、*13和*1502等位基因频率显著低于对照组(分别为0.4%vs4.6%,OR=0.093,95%CI0.012～0.720;7.6%vs13.7%,OR=0.516,95%CI=0.283～0.939;1.3%vs6.7%,OR=0.189,95%CI=0.055～0.648;2.7%vs8.5%,OR=0.298,95%CI=0.120～0.743);病例组与接触对照组间12个位点(密码子9、11、12、13、26、28、30、33、37、70、71、74)氨基酸密码子多态性分布频率差异有显著性。结论HLA-DRB1基因多态性可能是导致三氯乙烯药疹样皮炎个体易感性差异的原因之一。
Objective To investigate the relationship between genetic polymorphisms of human leukocyte antigen-DRB1 （HLA-DRB1） and the susceptibility to trichloroethylene（TCE）-induced severe generalized dermatitis. Methods A case-control study was conducted among 112 cases of TCE-induced severe generalized dermatitis and 142 healthy controls who were also exposed to TCE in the same workshops. Genotyping of HLA-DRB1 exon2 was performed by direct sequencing of polymerase chain reaction products. The frequencies distribution of allelic genotypes and cedon polymorphisms were compared between the cases and the controls, and odds ratio （OR） and 95% confidence interval （95% CI） were calculated. Results There were significant differences in the frequencies of allelic genotypes of HLA-DRB1 between the cases and the controls. The frequencies of DRB1^＊11501, ^＊ 1202 and ^＊ 04 in the cases were significantly higher than those in the controls （ 18. 8% vs 7.7% ,OR = 2. 748, 95% CI=1.587～4.761; 12.5% vs 6.0% ,OR=2.244,95% CI =1. 195～4.214; 17.4% vs 11.3% ,OR=1.660,95% CI = 1. 002 ～2. 750, respectively） , while frequencies of DRB1^＊ 0301,^＊ 0901, ^＊ 13 and ^＊ 1502 in the cases were significantly lower than those in the controls （0. 4% vs 4. 6% ,OR =0. 093,95% CI 0. 012 ～0. 720; 7.6% vs 13.7% ,OR = 0. 516,95% CI = 0.283 ～0.939;1.3% vs 6.7%,OR=0.189,95% CI=0.055～0.648; 2.7% vs 8.5%,OR= 0.298,95% CI=0.120～ 0. 743, respectively ）. There were significant differences in distributions of DRB1 cedon polymorphisms （cedon 9,11,12,13,26, 28,30,33,37,70,71,74） between two study groups. Conclusion The genetic polymorphisms of HLA-DRB1 might be one of the factors influencing the individual susceptibility to TCE-induced severe generalized dermatitis.
出处
《中国职业医学》
CAS
北大核心
2006年第5期337-340,共4页
China Occupational Medicine
基金
国家自然科学基金(No.30271115
No.30500403)
973项目资助(CB2002512903)
关键词
三氯乙烯
人类白细胞抗原DRB1
基因多态性
药疹样皮炎
Trichloroethylene
Human leukocyte antigen-DRB1
Gene polymorphism
Severe generalized dermatitis
