摘要
目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体在胰腺癌中的表达及意义。方法应用半定量RT-PCR法检测TRAIL受体(死亡受体DR4、DR5和诱骗受体DcR1、DcR2)mRNA在胰腺癌组织及正常胰腺组织中的表达。结果死亡受体DR4和DR5在所有胰腺癌组织和正常胰腺组织中均有表达,且在胰腺癌组织中的表达明显强于在正常胰腺组织中的表达(P<0.01)。诱骗受体DcR1和DcR2在所有正常胰腺组织中均有表达,而在胰腺癌组织中仅有18例表达DcR1,有20例表达DcR2;诱骗受体DcR1和DcR2的表达水平在胰腺癌和正常胰腺组织中差异无统计学意义(P>0.05)。胰腺癌组织中DR5的表达与肿瘤的分化程度和临床分期有关,分化程度越低,DR5的表达量越低,Ⅲ、Ⅳ期肿瘤DR5的表达显著低于Ⅰ、Ⅱ期(P<0.05)。DR4、DcR1及DcR2在胰腺癌组织中的表达与肿瘤的分化程度和临床分期无关(P>0.05)。结论①胰腺癌组织中普遍存在TRAIL受体的表达,并存在受体类型的表达差异,TRAIL基因受体在胰腺癌凋亡的调控机理中可能发挥重要作用。②胰腺癌组织中DR5的表达与肿瘤的分化程度及恶性程度相关;死亡受体DR4及诱骗受体DcR1和DcR2不能作为判断胰腺癌分化程度及恶性程度的指标。
Objective To investigate the expression and significance of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL） receptors in pancreatic cancer. Methods Thirty-two samples of pancreatic cancer tissue were collected from year 2002 to 2004. All of them were verified by histopathology and there were 9 cases of well-differentiated, 12 of moderately differentiated, and 11 of poorly differentiated, in which 12 cases were in the stage of Ⅰ or Ⅱ and 20 in the stage of Ⅲ or Ⅳ according to the TNM staging method. Eighteen normal pancreatic tissues were used as control group. The expressions of TRAIL receptors （death receptor 4, death receptor 5, decoy receptor 4 and decoy receptor 5） mRNA were assayed by semi-quantitive reverse transcription polymerase chain reaction （RT-PCR） in the pancreatic cancer tissues and the normal pancreatic tissues. Results The expressions of death receptor 4 （DR4） and death receptor 5 （DR5） were detected in all the pancreatic cancer tissues and the normal pancreatic tissues and the levels of DR4 and DR5 were significantly higher than those of the normal pancreatic tissues （P〈0.01）. Decoy receptor 1 （DcR1） and decoy receptor 2 （DcR2） were also expressed in normal pancreatic tissues, whereas DcR1 and DcR2 were only expressed in 18 and 20 pancreatic cancer tissues, respectively. However, there were no significant difference of the expression of DcR1 and DcR2 between the pancreatic cancer tissues and the normal pancreatic tissues （P〉0.05）. The expression level of DR5 in pancreatic cancer tissue was correlated with tumor differentiation and clinical stage, and the levels in stage Ⅲ and stage Ⅳwere significantly lower than those of stage Ⅰ and stage Ⅱ （P〈0.05）. The expressions of DR4, DcR1 and DcR2 were not correlated with tumor differentiation and clinical stage （P〉0.05）. Conclusion ①The expression of TRAIL receptors in pancreatic cancer tissues is prevalent, but the types of receptors expressed in different tissues were
出处
《中国普外基础与临床杂志》
CAS
2006年第5期521-523,530,共4页
Chinese Journal of Bases and Clinics In General Surgery
关键词
胰腺肿瘤
肿瘤坏死因子相关凋亡诱导配体受体
逆转录聚合酶链反应
Pancreatic neoplasm Tumor necrosis factor-related apoptosis inducing ligand receptor Reverse transcription polymerase chain reaction
