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水通道蛋白4在感染性脑水肿大鼠脑组织中变化的意义 被引量:5

Significance of Expression of Aquaporin-4 in Inflammatory Brain Edema in Infant Rats
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摘要 目的观察内毒素致幼年大鼠感染性脑水肿后脑组织水通道蛋白4(AQP4)表达的变化。方法将50只幼鼠随机分为内毒素组(40只)和对照组(10只)。建立内毒素致大鼠感染性脑水肿模型。于术后6、12、24、48 h处死动物,分别行脑组织含水量、血脑屏障通透率测定,以伊文思兰含量判定血脑屏障通透率,并应用免疫组织化学技术检测脑组织内AQP4表达水平。结果内毒素组脑组织含水量和伊文思兰(EB)水平明显高于对照组。在脂多糖注射后6 h时,幼鼠脑组织AQP4表达明显增加,12 h时达高峰,各时间点与对照组比较均有显著性差异(P<0.01)。结论感染性脑水肿后脑组织AQP4表达增加,且与脑组织含水量呈正相关。 Objective To observe the expression of aquaporin - 4(AQP4)in inflammatory brain edema by injecting endotoxin into infant rats. Methods Fifty infant rats were divided into endotoxin group( n = 40) and control group ( n = 10). Firstly, brain edema model was established by injecting endotoxin into infant rats. The brain tissue water content and Evens blue content were detected at 6, 12, 24, 48 h after injection, and immunohistochemistry was used to investigate AQP4 expression in brain tissue. Results Brain water content and Evens blue content were significantly higher in endotoxin group than that in control group. The level of AQP4 significantly increased at 6 h after injecting endotoxin, and the peak time of it was at 12 h. The differences between the level of AQP4 in endotoxin group and control group had statistic significance at each time point. Conclusions The level of AQP4 increase in inflammatory brain edema by endotoxin into infant rats. It has pasitive relationship with brain tissue water content.
出处 《实用儿科临床杂志》 CAS CSCD 北大核心 2006年第18期1225-1227,共3页 Journal of Applied Clinical Pediatrics
关键词 水通道蛋白4 脑水肿 内毒素 aquaporin - 4 brain edema endotoxin
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参考文献12

  • 1Itoh T,Rai T,Kuwahara M,et al.Identification of a novel aquaporin,AQP12,expressed in pancreatic acinar cells[J].Biochem Biophys Res Commun,2005,330(3):832-838. 被引量:1
  • 2Badaut J,Lasbennes F,Magistretti PJ,et al.Aquaporins in brain:Distribution,physiology,and pathophysiology[J].J Cereb Blood Flow Metab,2002,22:367-378. 被引量:1
  • 3Meng S,Qiao M,Lim L,et al.Correspondence of AQP4 expression and hypoxic-ischamemic brain edema monitored by magnetic resonance imaging in the immature and juvenile rat[J].Eur J Neurosci,2004,19 (8):2261-2269. 被引量:1
  • 4Guo Q,Sayeed I,Baronne LM,et al.Progesterone administration modulates AQP4 expression and edema after traumatic brain injury in male rats[J].Exp Neurol,2006,198 (2):469-478. 被引量:1
  • 5Taniguchi M,Yamashita T,Kumura E,et al.Induction of aquaporin-4 water channel mRNA after focal cerebral ischemia in rat[J].Brain Res Mol Brain Res,2000,78:131-137. 被引量:1
  • 6Papadopoulos MC,Saadoun S,Binder DK,et al.Molecular menchanisms of brain tumor edema[J].Neuroscience,2004,129:1011-1020. 被引量:1
  • 7禚志红,赵晓明,王怀立,高铁铮.巨噬细胞炎症蛋白2在脂多糖致大鼠脑水肿中的表达[J].实用儿科临床杂志,2005,20(4):346-348. 被引量:2
  • 8Tomas-Camardiel M,Venero JL,de Pablos RM,et al.In vivo expression of aquaporin-4 by reactive microglia[J].J Neurochem,2004,91(4):891-899. 被引量:1
  • 9Saadoun S,Papadopoulos MC,Krishna S,et al.Water transport becomes uncoupled from K+ siphoning in brain contusion,bacterial meningitis,and brain tumours:Immunohistochemical case review[J].J Clin Pathol,2003,56:972-975. 被引量:1
  • 10Papadopoulos MC,Verkman AS.Aquaporin-4 gene disruption in mice reduces brain swelling and mortality in pneumococcal meningitis[J].J Biol Chem,2005,280(14):13906-13912. 被引量:1

二级参考文献7

  • 1YU PL,Zhou PF,Yang YJ,et al. Brain edema model induced with typhoid endotoxin rabbits. In: Inaba Y. Klalzol. Spatz M. Brain Ede-ma[M]. Berlin :Springer Ver lag,1985:113- 116. 被引量:1
  • 2Frevert CW,Farone A, Danase H, et al. Functional characterization of rat chemokine macrophage inflammatory protein- 2[J ]. Inflammation, 1995,19(1 ): 133 - 142. 被引量:1
  • 3Schramm R, Thorlacius H. Staphylococcal exterotoxin B- induced acute inflammation is inhibited by dexamethasone: important role of CXC chemokines KC and macrophage inflammation protein 2 [ J ].Infect Immun, 2003,71 (5): 2542 - 2547. 被引量:1
  • 4Lldan F, Polat S,Oner A, et al. Effects of naloxone on sodium-and potassium- activated and magnesium - dependent adenosine - 5' -triphosphatase activity and lipid peroxidation and early ultrastructural findings after experimental spinal cord injury [J ]. Neurosurgery,1995,36 (4): 797 - 805. 被引量:1
  • 5Liu B,Du L,Hong JS. Naloxone protects rat dopaminergic neurons against inflammatory damage through inhibition of microglia activation and superoxide generation [J ]. J Pharmacol Exp Ther, 2000, 293(2) :607 - 617. 被引量:1
  • 6Liao SL, Chen WY, Raung SL, et al. Neuroprotection of naloxone against ischemic injury in rats: role of mu receptor antagonism [J ].Neurosci Lett ,2003,345(3): 169 - 172. 被引量:1
  • 7王怀立,芦军萍,赵晓明,高东培,罗强,高铁铮.细胞间黏附分子-1在脂多糖致大鼠脑水肿中的表达[J].实用儿科临床杂志,2003,18(3):179-181. 被引量:5

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