摘要
Objective ： To investigate the therapeutic effect ofBc1-2 fusion protein on apoptosis in brain following traumatic brain injury. Methods： Bcl-2 gene was cloned by RT-PCR. Bd-2 and EGFP genes were linked together and inserted into pAdeno-X vector. This recombinant vector was packaged into infectious adenovirus in HEK293 cells. Ninety Wistar rats were assigned randomly into experimental group （n=45） and control group （n=45）. All rats were subjected to traumatic brain injury. Then recombinant adenovirus （ for experimental group） or saline （ for control group） was injected into the traumatic brain. The expression of Bcl-2 fusion protein was investigated by Western blotting, immunohistochemistry and fluorescence microscopy. Apoptosis in the injured brain was studied by TUNEL. Animals＇ behavior capacity was evaluated by tiltboard test. Results ： In the experimental group, many fluorescent cells were found around the traumatic locus,which were also proven to be Bcl-2-positive by immunohistochemistry. On the contrary, few Bcl-2-positive cells and no fluorescent cell were detected in the control group. Bcl-2 expression of experimental group was much higher than that of control group, which was illustrated by Western blotting. The apoptosis index of experimental group was 0. 027 ± 0. 005, and that of control group was 0. 141 ± 0.025 （ P 〈 0.01 ）. Two weeks after injury, animals of the experimental group behaved better than those of the control group. Conclusions ： A recombinant adenovirus vector expressing Bcl-2 fusion protein has been constructed. Bcl-2 fusion protein can suppress apoptosis and promote cell survival. Moreover, the behavior recovery of the injured animal is promoted. Bcl-2 fusion protein provides a way to track the target cells in vivo.
