摘要
通过剥蕾反复授粉克服欧洲甜樱桃与毛樱桃种间杂交障碍,采用胚培养的方法克服了远缘杂种在自然状态下胚败育的情况发生,获得了以甜樱桃先锋为母本,以毛樱桃为父本的杂种G3.本实验利用AS-PCR技术将S等位基因作为樱桃砧木辅助育种中的分子标记,对获得的远缘杂种在苗期进行S基因型鉴定.结果表明,G3的确是远缘杂交的新种质,RAPD分析显示G3同时具有母本和父本的特征随机扩增DNA片段,进一步证实了AS-PCR方法的可靠性.通过远缘杂交的育种能够有效地扩展樱桃砧木的遗传背景,另一方面利用分子标记的手段在苗期即可进行预先选择和鉴定.
Incompatibility of interspecies cross between sweet cherry and Cerasus tomentosa was overcome by successive hand pollination, and the new germplasm G3 was obtained by using embryo culture technology to overcome the embryo abortion of distance hybridization. The G3 S-genotype was identified in seedling using AS-PCR, and the self-incompatibility gene of the parent was used as the molecular marker to validate the pedigree of hybrid G3. The RAPD analysis further demonstrated that G3 share characteristic RAPD fragments of both parents. This result suggested that AS-PCR was an effective and credible method to identify the progeny of interspecies hybrid in Prunus. And the cross could be a promising way to enhance the genetic background. DNA molecular marker realized the identification and pre-selection in the seedling stage. So the developing breeding program would be accelerated by the new way.
出处
《中国科学院研究生院学报》
CAS
CSCD
2006年第5期671-675,共5页
Journal of the Graduate School of the Chinese Academy of Sciences
