摘要
目的:通过MDM2反义寡核苷酸(MDM2-AS)抑制白血病细胞系K562和HL-60中MDM2的表达,研究其化疗增敏作用。方法:细胞分为对照组、AS组、顺铂组和联合处理组,分别单独或联合MDM2-AS转染细胞,以RT-PCR和WESTERNBLOT检测MDM2的表达;以单细胞凝胶电泳检测细胞DNA损伤;以流式细胞仪检测处理后各组细胞的凋亡率。结果:经MDM2-AS转染后细胞的MDM2表达明显受抑制(P<0.05),而经过MDM2-AS与顺铂联合处理细胞,K562和HL60细胞凋亡率明显增加(P<0.01),与顺铂处理组相比亦明显增加(P<0.05)。结论:以MDM2-AS反义封闭抑制MDM2的表达,可以增加白血病细胞的化疗敏感性,提示这一方法在白血病治疗中的广泛应用前景。
Objective： Chemotherapeutic sensitization of MDM2 antisense Oligonuleotides （MDM2-AS） was studied in leukemia cells including K562 and HL60. Method： All the cells were divided into control group, AS group, Cisplatin group, and integrated treatment group, into which MDM2-AS is transfected solely or integrated. Western blot and RT-PCR were used to detect MDM2 expression; DNA damage was evaluated by Comet assay; apoptosis rate of all groups were detected by FACS. Result： MDM2 expression was inhibited obviously after MDM2-AS transfection （ P〈0.05）. Apoptosis rate of cells which were treated with MDM2-AS and Cisplatin integrated increased obviously （ P〈0.01）, and also increased obviously than that of Cisplatin treatment group cells （ P〈0.05）. Conclusion： MDM2 inhibition with MDM2-AS transfection, can present chemotherapeutic sensitization of leukemia cells, which indicates wide use of this treatment in leukemia chemotherapy.
出处
《临床血液学杂志》
CAS
2006年第5期294-296,299,共4页
Journal of Clinical Hematology
基金
湖北省自然科学基金资助(No:2005ABA150)
