摘要
以微型番茄M icro-T om核基因组DNA为模板,利用PCR方法扩增编码有色体特异性的番茄红素β-环化酶的B基因编码区,并将B基因与在高等植物中组成型表达的C aM V 35S启动子融合,构建成双元载体pL e-BZN,导入农杆菌,利用农杆菌介导的遗传转化方法转化到微型番茄M icro-T om中,通过抗性筛选和PCR鉴定,成功获得了多株转基因微型番茄,为获得富含β胡萝卜素的转基因微型番茄奠定了基础.
In order to improve the Q-carotene content in tomato fruits, full length of B gene successfully isolated by using PCR method with Micro-Tom genomic DNA as template, then CaMV 35S promoter：： B fusion gene was constructed in binary vector pCAMBIA-1. The so-called pLeBZN plasmid was transformed into Micro-Tom via Agrobacterium-meed transformation. Antibiotics screening and the chimeric gene amplification from genomic A identified several transgenic plants. The alteration of β-carotene and lycopene contents in lsgenic Micro-Tom fruits is to be checked.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期103-107,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津市应用基础研究计划重点项目(05YFJZJC00800)
