摘要
目的探讨不同病期哮喘患儿血清IL-12、IL-4水平、细胞免疫功能及其相互关系。方法随机选择哮喘患儿50例。婴幼儿哮喘13例,儿童哮喘37例。发作期30例,经吸入激素治疗后缓解期患儿20例。健康对照组22例。IL-12、IL-4测定采用酶联免疫双抗体夹心法(ELISA)。采用碱性磷酸酶抗碱性磷酸酶桥联酶标试验(APAAP)检测T细胞亚群,采用郭氏法检测红细胞免疫功能。结果1.哮喘患儿发作期、缓解期和对照组IL-12水平分别为(24.44±13.26)、(42.30±12.65)、(44.68±28.28)ng/L,3组比较有显著性差异(F=8.92P<0.01),发作期明显低于其他两组,缓解期与对照组无显著性差异。2.IL-4在发作期、缓解期、对照组阳性率分别为70%、35%、9%,发作期明显高于缓解期(χ2=5.96P<0.05),缓解期仍高于对照组(χ2=4.17P<0.05)。3.哮喘患儿与健康儿童相比,CD3(t=3.18P<0.01)、CD4(t=5.51P<0.01)、CD8(t=10.38P<0.01)均降低,CD4/CD8升高(t=4.28P<0.01),RBC-C3bR降低(t=4.65P<0.01),RBC-IC增高(t=10.22P<0.01)。4.IL-4与IL-12呈显著负相关(r=-0.43P<0.05),与RBC-IC呈显著正相关(r=0.49P<0.05)。结论哮喘患儿存在Th1/Th2类细胞因子失衡和细胞免疫功能紊乱状态,哮喘患儿缓解期IL-12水平恢复至接近健康儿童,IL-4水平仍高于健康儿童。应坚持长期抗感染治疗。
Objective To study the changes of serum interleukin（IL） - 4, IL- 12 and correlation with cellular immunity in children with asthma of different stages. Methods Fifty asthmatic children were randomly selected, including 30 cases in attack stage （group A） and 20 cases in remission stage （group R）. At the same time, 22 healthy children were studied as normal controls （group N）. The levels of IL- 12 and IL - 4 , T cells subgroups and erythrocyte immunity were detected. Results 1. Serum IL - 12 levels were （24.44 ± 13.26）, （42.30 ± 12.65）, （44.68 ± 28.28） ng/L in group A, R and N, respectively, There was significant difference in three groups （F = 8.92 P〈 0.01 ）. The level of IL- 12 in group A was the lowest, and there was no significant difference in other two groups. 2. The positive rate of IL -4 were 70% ,35% ,9% in group A, R and N, respectively, which had significant difference （x^2 = 20.16 P〈 0.01 ）. The positive rate of IL- 4 in attack stage was markedly higher than that in remission stage（x^2 = 5.96 P〈 0.05） ; there was significant difference between group R and N （x^2 = 4.17 P 〈 0.05）. 3. Compared with healthy children, CD3, CD4, CD8, RBC - C3bR decreased（P〈0.01） ,but CD4/CD8 （P〈0.01） and RBC- IC（P〈0.01） increased in group A,R,N. 4. IL-4 had negative relationship with IL- 12 （r= -0.43 P〈0.05） ,and had positive relationship with RBC- IC （r=0.49 P〈0.05）.There was no relationship between all the others. Conclusions There are imbalance of rate of Thl/Th2 which related to disorder of cellular immunity in children with asthma. Serum IL- 12 level in remission children almost returns to normal, but IL- 4 level showes significant difference compared to normal group, which suggests that corticosteriods shall be used consistently.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第16期1083-1084,共2页
Journal of Applied Clinical Pediatrics
