摘要
目的:探索用前脑缺血法建立老龄大鼠脑源性多器官功能障碍综合征模型的方法。方法:实验于2005-10/12在解放军兰州军区总医院动物实验室进行。①取192只健康老龄Wistar大鼠(24月龄),抽签法随机分为对照组6只,假手术组6只,缺血20,30,40min组各60只,根据取材时间后3组又随机分为术后1,3,5d组3个时相点,每时相点20只。②对照组不干预;假手术组麻醉后分离双侧颈总动脉,不夹闭颈总动脉;其他3组通过夹闭双侧颈总动脉致大鼠前脑急性缺血(夹闭时间分别为20,30和40min),建立老龄大鼠脑源性多器官功能障碍综合征模型。③观察术后大鼠的症状和生命体征,计算死亡率和多器官功能障碍综合征的发生率。分别在术后1,3,5d时相点,将各组未死亡的大鼠全麻后采集颈动脉血,检测血气、血清丙氨酸转氨酶、总胆红素、尿素氮、肌酐、肌酸激酶,采血后处死动物,观察大鼠主要脏器的病理变化。对照组和假手术组在1d时间点检测。结果:142只大鼠进入结果分析。①死亡率:缺血40min组高于缺血20,30min组(45%,15%,23%,P<0.05)。②多器官功能障碍综合征的发生率:缺血20min组低于缺血30,40min组(15%,23%,45%,P<0.05);在缺血30,40min组,术后1d高于术后5d(56%比20%;64%比17%;P<0.05)。③大鼠急性脑缺血再灌注后,肺、肝、肾、心脏功能有不同程度损伤,与对照组和假手术组比较,表现为动脉氧分压下降,丙氨酸转氨酶、肌酸激酶、总胆红素、肌酐升高;在缺血30和20min组内,术后1,5d间差异显著(P<0.05)。结论:急性前脑缺血30min再灌注1d老龄大鼠脑源性多器官功能障碍综合征发生率较高。
AIM： To establish a model of acute forebrain ischemia complicated with multiple organ dysfunction syndrome in elderly rats. METHODS： The experiment was conducted in the Laboratory for Animal, General Hospital of Lanzhou Area Command of Chinese PLA from October to December 2005. ①A total of 192 healthy elderly Wistar rats （aged 24 months） were randomly divided into five groups： control group （n=6）, sham operation group （n=6）, and 20, 30 and 40 minutes ischemia groups （n=60） including days 1, 3 and 5 time points with 20 in each time phase. ②Control group did no receive intervention. Bilateral common carotid artery was isolated, but not occluded in the sham operation group. Forebrain acute ischemia was induced by occluding bilateral common carotid artery （20, 30 and 40 minutes for occluding, respectively） to establish cerebrogenic multipie organ dysfunction syndrome in elderly rats. ③Changes of symptom and signs were recorded after operation. Changes of function and morphology of vital organs were detected. At days 1, 3 and 5 after operation, carotid arterial blood were collected after the whole anesthesia of living rats in each group to examine blood gas, blood alanine transaminase, total bilirubin, urea nitrogen, creatinine and creatine kinase, and then the animals were killed. Pathological changes of main organs of rats were observed. The control group and sham operation group received detection at day 1. RESULTS： A total of 142 rats were involved in the result analysis.① Mortality rate： It was higher in the 40 minutes ischemia group than the 20 and 30 minutes ischemia groups （45%, 15%,23%,P 〈 0.05）.②Incidence rate of multiple organ dysfunction syndrome： It was lower in the 20 minutes ischemia group than the 30 and 40 minutes ischemia groups （15%, 23%,45%,P 〈 0.05）. It was higher at day 1 after operation than at day 5 in the 30 and40 minutes ischemia groups （56% vs 20%;64% vs 17%;P 〈 0.05）. ③After acute cerebral ischemia/reperfusion of rats, lung, live
出处
《中国临床康复》
CSCD
北大核心
2006年第34期73-75,共3页
Chinese Journal of Clinical Rehabilitation
