摘要
背景:急性脑血管病导致的多器官功能障碍综合征(multipleorgandysfunc-tionsyndrome,MODS)在临床上是常见的,而对其发生的机制研究较少。目的:探讨脑缺血模型内毒素受体CD14mRNA在肺、肝、肠和肾组织的表达变化规律及并发MODS的机制。设计:随机对照的前瞻性研究。地点和对象:在山东大学齐鲁医院实验动物中心将54只Wistar大鼠分为正常对照组、假手术组及缺血后5个亚组(12,24,36,48,72h组)。干预:实验人员采用两侧颈总动脉阻断法建立前脑缺血模型。主要观察指标:观察术后动物的一般情况,包括意识、精神状态、行动等。检测麻醉后大鼠的肛温和呼吸频率,测定血常规、肝功、肾功。观察缺血后各脏器大体标本的形态变化,检测各脏器组织CD14mRNA的表达。结果:大鼠急性前脑缺血后MODS发生率为53%;缺血后12,24,36,48,72h时相点动物肺、肝、肠和肾组织均有不同程度的损害,以肺脏和小肠改变为著;缺血后12h各脏器组织CD14mRNA表达升高,24～36h达高峰,48h后下降,以肺脏变化最显著(P<0.001);正常对照组和假手术组中各脏器组织CD14mRNA均有不同程度的表达,其中两组肺脏CD14mRNA的表达有显著性差异(P<0.01)。结论:脑缺血后各脏器组织CD14mRNA的异常表达和病理改变为肠道内毒素易位和内毒素血症的发生提供条件。
BACKGROUND:It is very common clinically that the acute cerebrovascular disease leads to multiple organ dysfunction syndrome(MODS),but the studies on its mecha nism are rare. OBJECTIVE:To discuss the expressive disciplinarian of endotoxin receptor,CD14m RNA in lung,liver,intestines and kidney tissues and the mechanism of complicatin g disease of MODS in cerebral ischemia model. DESIGN:A radomized controlled study. SETTINGS and PARTICIPANTS:Fifty-four Wistar rats were randomly allocated into normal control,sham-operation and ischemia group including 5 sub-groups(12,24 ,36,48 and 72-hour sub-groups) in the experimental animal centre of Qilu Hospi tal Affiliated to Shandong University. INTERVENTIONS:Forebrain ischemia model was established by interdiction in both sides of common carotid arteries. MAIN OUTCOME MEASURES:General postoperative conditions were observed including consciousness,mental status,and behaviour,etc.The anal temperature and respirat ory frequency after anaesthesia was measured,and the routine blood test,liver fu nction and kidney function were tested.The morphological changes in very organ s pecimen after ischemia were observed.The expression of CD14mRNA in each organ wa s assayed. RESULTS:The incidence of MODS was 53%after acute forebrain ischemia in rats.T here were damages in lung, liver, intestines and kidney tissue with different de gree at 12,24,36, 48 and 72 hours after ischemia,in which the most obvious chang es were in lung and small intestines.The expression of CD14mRNA in each organ at 12 hours after ischemia increased and hit its peak at 24 to 36 hours and decrea sed after 48 hours,in which the most significant change was in lung(P< 0.01).The re were expressions of CD14mRNA in different degree in each organ in normal cont rol and sham-operation group, in which there was significant difference in the expression of CD14mRNA in lung between two groups(P< 0.01). CONCLUSION:The abnormal expression of CD14mRNA in every organ and the patholog ical changes after cerebral ischemia
出处
《中国临床康复》
CSCD
2004年第16期3172-3173,共2页
Chinese Journal of Clinical Rehabilitation
