摘要
目的研究低剂量照射(LDR)对荷人小细胞肺癌(NC I-H446)裸小鼠移植瘤细胞凋亡相关基因蛋白表达的影响。方法35只荷人小细胞肺癌(NC I-H446)裸小鼠,随机分为7个实验组,假照组(0 mGy);D1组(75 mGy);D2组(4 Gy);D1-12 h+D2组(D1照射后12 h予以D2);D1-24 h+D2组(D1照射后24 h予以D2);D1-48 h+D2组(D1照射后48 h予以D2);D1-72 h+D2组(D1照射后72 h予以D2)。各实验组不同方式照射后4 d,荷瘤裸鼠全部处死,采用免疫组化技术检测荷瘤组织的P53、Bcl-2、Bax的蛋白表达水平。结果D1组照射后,小细胞肺癌细胞的p53、Bcl-2的蛋白表达呈下降趋势,Bax蛋白表达呈上升趋势,但与假照组比较,无明显差异(P>0.05);D2组、D1+D2组的p53、Bcl-2蛋白表达明显减少,Bax蛋白表达明显增多,与假照组比较差异显著(P<0.05);而D1+D2组与D2组比较,差异显著(P<0.05),以D1+D2组p53、Bcl-2蛋白的表达减少、Bax蛋白的表达增多更为明显。结论低剂量辐射可能在一定程度上下调了小细胞肺癌细胞的p53、Bcl-2的蛋白表达,上调了Bax蛋白的表达,同时对其后的大剂量照射有协同作用。
Objective To study the effect of low dose radiation （LDR） on expression of apoptosis-related gene mRNA of transplantation tumor tissues of human small cell lung carcinoma （NCI-H446） -bearing nude mice. Methods Tumor （NCI-H446） -bearing nude mice transplantation tumor tissues were radiated by whole body X ray to detect the expression of mRNA of p53, bel-2 and bax of transplantation tumor tissues by situ hybridization. Results The transcription level of p53 and bax mRNA were slightly up-regulated and bel-2 mRNA was slightly down-regulated in D1 （75 mGy）group and D2 （4 Gy）group, but without significant difference comparing with sham-radiated group （0 mGy） （P〉0.05） ; while those in D1 ＋D2 groups were significantly different with those of sham-radiated group （P〈0.05）. Conclusions LDR could up-regulate the expressions of p53 and bax mRNA and down-regulate bel-2 mRNA expression to some extent, and maybe has synergic effect with high dose radiation.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第8期1075-1077,共3页
Chinese Journal of Gerontology
基金
吉林省科技发展计划资助课题(200505183)
关键词
低剂量照射
人小细胞肺癌(NCI-H446)
裸小鼠
凋亡
免疫组化
Low dose radiation （LDR）
Human small cell lung carcinoma （NCI-H446）
Nude mice
Apoptosis
Situ hybridization
C, ene
