摘要
用30 L发酵罐研究了抗真菌肽枯草杆菌工程菌B.subtilisDB1342(pSC18 66)的高密度发酵工艺.通过补料和蔗糖诱导24 h后,工程菌DB1342(pSC18 66)生物量的A600值达到53,目的蛋白分泌量为25 mg/L.发酵液经硫酸铵沉淀浓缩后,过DEAE-Sephadex A 25离子交换柱和Sephadex G 75分子筛层析进行纯化,产物纯度达到90%以上.抑制真菌实验结果显示:纯化产物能抑制白假丝酵母、镰刀菌和链格孢霉菌的生长.
The high cell density of B. subtilis DB 1342 （pSC18 - 66） strain harboring antifungal peptide was cultured in a 30 liter fermentor. The biomass A600 of DB1342（pSC18-66） reached 53, the secreted CGA18-66 was 25 mg/L by feed-batch fermentation and sucrose induction for 24 hours. The fermentation supernatant was concentrated with ammonium sulfate, and the expressed product was purified to 90% with DEAE-sephadex A-25 anion exchange chromatography and Sephadex G-75 gel filtration.The purified product specifically inhibited the proliferation of Candida albican , Alternaria sp . and Fusarium sp . at 4 μ mol/L.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2006年第4期19-22,共4页
Journal of Henan University of Technology：Natural Science Edition
基金
广东省自然科学基金资助项目(011207)
关键词
抗真菌肽
枯草杆菌
高密度发酵
纯化
生物活性
antifungal peptide
Bacillus subtilis
high density culture
purification
bioactivity
