摘要
用甲醇：氯仿（２：１）抽提，硅胶柱、薄层层析法分离纯化自新鲜牛心中提取心磷脂，经硅胶板层析和高效液相色谱分析，其比移值（Ｒｆ）与峰形与心磷脂标准品完全一致。用此心磷脂包被反应板建立了检测抗心磷脂抗体（ＡＣＡ）的间接ＥＬＩＳＡ法。酶结合物采用抗人γ、抗α、抗μ链抗体的Ｆ（ａｂ＇）＿２片段。结果表明全身性红斑狼疮、类风湿关节炎、血小板减少症和反复自然流产患者ＡＣＡ均有较高的检出率（２５．０％～３３．８％）。
A crude phospholipid fraction was extracted from fresh beef heart by methanol-chloroform(2: 1 ).Further isolation and purification of cardiolipin was achived by silica gel column,thin-layer chromatography.It was detected with silica gel thin-layer chromatography and high performance liquid chromatography(HPLC),and indicated that it′s Rf value and peak profile was completely concordant with reference cardiolipin (Sigma).With it coated microtitre plates ,had established indirect ELISA for detection of anticardiolipin antibodies,and adopted the HRP conjugated F (ab′)2 fragments of antihuman γ、α、μ chain antibody.The results indicated that SLE、RA 、thrombocytopenia and recurrent spontaneous abortion (RSA)groups had a higher positive rate of anticardiolipin antibodies(25.0%～33.8%).
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1996年第6期362-365,共4页
Chinese Journal of Immunology
关键词
心磷脂
抗心磷脂抗体
红斑狼疮
自然流产
Cardiolipin Anticardiolipin antibody SLE Recurrent sponta neous abortion
