摘要
目的:采用实时荧光定量RT-PCR和cDNA基因芯片2种技术分析食管癌细胞基因表达的特征。方法:应用cDNA基因芯片筛选15例食管癌基因表达谱,用实时荧光定量RT-PCR验证其中16个基因的表达变化。结果:实时荧光定量RT-PCR和cDNA基因芯片技术对16个基因的检测结果一致,不仅变化方向相同,而且表达值非常接近。结论:cDNA基因芯片和实时荧光定量RT-PCR分析基因表达变化都是可信的,基因芯片筛选基因表达谱具有高通量大规模的特点,而实时荧光定量RT-PCR则适合单个基因表达变化的研究,两者互为补充和印证。
Aim ： To compare the gene expression using real time RT-PCR and cDNA microarray in the human esophageal squamous cell carcinoma （ESCC）. Methods：The gene expression profile was screened by c DNA microarray in 15 cases of ESCC, and 16 genes of which were confirmed by real time RT-PCR. Results： The identical direction and almost identical genes expression of mRNA detected by real time RT-PCR was accorded with those by cDNA microarray. Conclusion ：cDNA microarray and real time RT-PCR techniques are faithful in the analysis of genes expression, cDNA microarray possesses highthroughput characteristic and real time RT-PCR is applicable to the analysis of target gene expression.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2006年第5期841-843,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目0624410100
0624410105
0624410096
