摘要
目的：探讨建立人眼眶横纹肌肉瘤动物模型的方法。方法：培养人胚胎型横纹肌肉瘤细胞株RD细胞，取对数生长期细胞分别接种于4周龄SCID鼠和^60Co照射（1Gy）后1d的BALB／c裸小鼠；选用成瘤BALB／c裸小鼠的瘤块移植入另一批4周龄BALB／c裸小鼠胁部皮下（组织块接种法）。观察各组动物肿瘤生长情况，绘制肿瘤生长曲线；肿瘤组织行常规病理H-E染色并镜检。结果：^60Co照射后1d裸小鼠成瘤时间约6～8周，SCID鼠组约3～4周，组织块接种法移植2周后裸小鼠瘤体较明显，3组动物的成瘤率均为lOO％；成功绘制出肿瘤生长曲线，肿瘤均呈进行性生长；病理检查示各组移植性横纹肌肉瘤与人体肿瘤标本所见类似。结论：成功建立了裸小鼠和SCID鼠人眼眶横纹肌肉瘤模型，组织块接种法可以缩短BALB／c裸小鼠成瘤时间，为进一步研究人眼眶横纹肌肉瘤奠定了基础。
Objective：To establish a mouse model of human orbital rhabdomyosarcoma（RD）. Methods： RD cells were cultured and implanted subcutaneously into the costal regions of nude mice and severe combined immunodeficiency （SCID） mice （4 weeks old）. The nude mice were radiated by ^60Co 1 day before the injection. The rhbdomyosarcoma tissue masses were harvested from nude mice and were subcutaneously implanted into the costal regions of another group of BALB/c mice （tumor tissue mass implantation）. The tumor growth was observed in all groups and the growth curves were ploted. H-E staining was used to examine pathology of the tumor tissues. Results： Solid tumors were palpable 6-8 weeks after implantation in ^60Co radiated nude mice and about 3-4 weeks after implantation in SCID mice. The solid tumors were obviously seen in the nude mice 2 weeks after tumor tissue mass transplantation. The tumor forming rates were 100% in all groups. The tumor growing curves were successfully ploted and the tumors had a progressive growth. Pathological findings of the tumor tissue were similar to those of human orbital rhabdomyosarcoma. Conclusion： The human orbital rhabdomyosarcoma models have been successfully established in nude mice and SCID mice. Tumor tissue mass implantation can shorten the tumor forming time in BALB/c nude mice. This research lays a foundation for further study or human orbital rhabdomyosarcoma.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第8期902-904,共3页
Academic Journal of Second Military Medical University
基金
上海市科委专项基金(0352nm114)~~
关键词
模型
动物
眼眶
横纹肌肉瘤
models, animal
orbital
rhabdomyosarcoma
