摘要
用索氏提取法提取山楂叶中黄酮化合物。以芦丁为标准品,对其进行络合显色,在最大吸收峰500nm处测定提取物中总黄酮的含量。山楂叶中总黄酮的含量以芦丁计算为9.16%,平均加样回收率为98.53%。方法简便、快速、重现性好,可作为检测山楂叶中黄酮含量的一种手段。
The flavones compounds were obtained by soxhlet extraction method. Rutin was reference standard and the concentration of flavones was determined by UV spectroscopy at the wavelength of 500nm after the cxtractant reacted with coloring agents. The flavones content in samples was 9. 16% calculated by rutin,and the recovery rate was 98. 53% respectively. The results show that the method is suitable for simple,rapid and reproducible analysis of flavones in hawthorn leaves.
出处
《光谱实验室》
CAS
CSCD
2006年第4期755-757,共3页
Chinese Journal of Spectroscopy Laboratory
关键词
分光光度法
山楂叶
黄酮
Spectrophotometry,Hawthorn Leaves,Flavones.
