摘要
通过土壤浸出液培养基和菌间相互拮抗试验，筛选并构建出由能拮抗土传病原菌的拮抗菌系和与之协同、快速繁殖的营养菌系组成的复合菌剂。复合菌剂在25—40℃，pH=6．0-8．0条件下生长良好。对番茄青枯病的平板拮抗试验的结果表明，复合菌剂对番茄青枯病病原菌、病土及番茄青枯病株中的微生物均有明显拮抗作用。盆栽试验结果显示，施入复合菌剂，可延缓病土的青枯病发病时间8-19d并减少发病株数2—3株，防效明显。
The present paper intends to report the primary results of the authors＇ research on the biological function of the composite microbial agent in preventing and controlling the plant disease bacterial wilt of tomato. As is known, the plant bacterial wilt of tomato is one of most serious soilborne diseases influencing the regular growth of tomato and, therefore, has become a hot topic in the research area. To solve the problem, endophtic and biocontrol bacteria and avirulent strains of Ralstonia solanacearum had once been used to control the bacterial wilt years ago, yet their full potential to soilborne microbial proved to be limited for the purpose. To overcome the limit, this paper initiated to adopt a so-called complex microbial agent consisting of antagonistic microbial group to soilborne disease and a nutritional microbial group that may help to quickly reproduce and cooperate with the antagonistic microbial as cultural media of soil extraction and mutual antagonistic test. The agent can grow properly under the condition of 25 - 40 ℃ and pH of 6.0 - 8.0. The result of our agar medium antagonistic test has shown that the composite agent is able todelay and inhibit tomato＇s bacterial wilt （Ralstonia solanacearum） and the pathogens existing in the continuous cropping soll and tomato plant disease. As the results of our pot experiments show, it can delay the disease happening by 8 to 19 days and reduce 2 to 3 diseased plants, though its full effect for controlling the disease has yet to be further observation and study,
出处
《安全与环境学报》
CAS
CSCD
2006年第4期27-30,共4页
Journal of Safety and Environment
基金
国家863计划资助项目(2001AA246023)
关键词
园艺学
番茄青枯病
复合菌剂
生物防治
horticulture
tomato bacterial wilt
complex microbial agent
biological control
