摘要
目的研究细胞黏附分子在高容量血液滤过(HVHF)干预多器官功能障碍(MODS)猪模型过程中的表达变化情况。方法18头实验动物被随机分为2组：模型组(M组)实施失血性休克+内毒素注射“二次打击”,而治疗组(HF组)在二次打击后接受HVHF干预。检测两组中性粒细胞(PMN)表面CD11b、L-选择素(selectin)的表达和血浆中可溶性E选择素(sE-selectin)、可溶性细胞间黏附分子(sICAM-1)的浓度。结果HVHF治疗开始后,HF组CD11b表达出现下降,在各个时间点与M组比较,差异有统计学意义P＜0.01；治疗组中L-selectin的表达在治疗后24、48h处表达高于M组的表达(P＜0.05)。治疗开始后sE-selectin浓度就出现明显下降。治疗后24h与M组比较差异有统计学意义(P＜0.01),到治疗后48h(P＜0.05)和72h(P＜0.01)已经高于M组水平；治疗后24h,HF组中sICAM-1水平低于M组(P＜0.05),此后维持于较高水平,至治疗后72 h高于M组(P＜0.01)。结论HVHF可以间接、双向调节MODS发生过程中细胞黏附分子的表达水平以及PMN、内皮细胞的活化状态。
Objective To investigate the effects of high volume hemofiltration （HVHF） on cell adhesion molecules in multiple organ dysfunction syndrome （MODS）. Methods Pigs were randomized into two groups： group M （n = 9） as a control group, receiving ＂two-hit＂ injury only;group HF （n = 9） as a treatment group receiving HVHF. The expression of CD11b and L-selectin in neutraphils was detected by using flow cytometry and serum concentrations of soluble E-selectin and intercellur adhesion molecule-1 （ICAM-1） determined by using enzyme-linked immunoabsordent assay （ELISA）. Results HVHF atteunuated significantly the increase of neutrophil CD11b and the levels of CD11b on PMN in HF group were significantly lower than in group M after HVHF intervention （ P 〈 0.01 ）. Yet, neutrophil L-selectin remained relatively stable in HF group, but significantly higher than in the untreated group at 24 and 48 h following HVHF （P 〈 0.05）. At 24th h after treatment, plasma sE-selectin concentrations in HF group were lower significantly than in group M （P 〈 0.01 ）. The levels of sE-selectin were higher than those in controls at 48 h （P〈0.05） and 72 h （P〈0.01） after treatment. The levels of sICAM-1 in treatment group were lower siginificantly than in controls at 24 h after HVHF （P 〈 0.05）. However, the concentration of sICAM-1 in group HF was still higher significantly than in controls at 72 h after HVHF （P 〈 0.01）. Conclusion HVHF could indirectly and bidirectionally regulate the expression of cell adhesion molecules and the activation of PMN and endothelial cells in the process of MODS.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第8期1000-1002,共3页
Chinese Journal of Experimental Surgery
基金
全军医药卫生科研基金(01L006)
关键词
多器官功能障碍综合征
血液滤过
黏附分子
Multiple organ dysfunction syndrome
Hemofiltration
Adhesion molecule
