摘要
综述了手性配合基交换色谱法通常采用三种手性相系统中的流动相添加剂方法。主要内容有：（Ａ）手性配合基交换机制，给出了描述对映体对在色谱系统中的保留时间和分离选择性的公式，包括手性选择剂在固定相和流动相中的各种不同情况。公式表明整个色谱往系统的对映体选择性不同于溶液中所存在的选择剂与被选择物作用的情况；（Ｂ）影响配合交换的参数，讨论了金属离子、金属离子／配位体比率、金属离子络合物浓度、固定相、流动相ｐＨ、洗脱顺序、有机调节剂、离子对试剂、流动相离子强度、温度、立体选择性和手性交互识别；（Ｃ）应用：列举了一些手性流动相法分离对映体的例子。
Chiral ligand-exchange chromatography(CLEC),in which three types of chiral LC systems are generally employed,is revieved with emphasis on the chiral mobile phase. The main points include : (A) Mechanism of chiral ligand-exchange chromatography. Equations are given describing the retention and separation selectivity of two enantiomeric species in a chromatographic system containing a chiral selector in the stationary and/or in the mobile phase. It is shown that the total column enantioselectivity generally differs from the enantioselectivity of the selector-selectand interaction in solution. (B)Parameters affecting ligand exchange. Twelve factors are disscused including metal ions, metal/ligand ratio, concentration of metal/complex, stationary phase, eluent PH, elution order,organic medifier, ion-pair reagent, ion strength of mobile phase, temperature,stereoselectivity, and mutual recognition. (C) Applications. Some enantiomers separated by CMPs of CLEC are presented as examples.
出处
《色谱》
CAS
CSCD
北大核心
1996年第5期354-359,共6页
Chinese Journal of Chromatography
基金
国家和浙江省自然科学基金
关键词
手性配合基
交换色谱法
流动相添加剂
chiral ligand-exchange chromatography,mobile additive, separation of enantiomers
