摘要
目的通过检测核小体结合蛋白1(NSBP1)在正常前列腺(NP)、良性前列腺增生(BPH)、前列腺癌(PCa)组织中的表达,探讨其与前列腺疾病发生发展的关系。方法采用Western印迹方法检测NSBP1在10例NP组织、15例PCa组织中的蛋白表达差异;采用免疫组织化学方法检测19例NP组织、26例BPH组织、40例PCa组织中NSBP1的表达水平。结果NSBP1在PCa组织中蛋白表达高于在NP组织中的表达,差异有统计学意义(t=37．308,P<0．01)。3种组织中NSBP1的阳性及弱阳性表达率为56．5％(48／85),其中NP、BPH、PCa组织中分别为36．8％(7／19)、34．6％(9／26)、80．0％(32／40)。PCa组织中NSBP1表达水平明显高于NP及BPH组织(t=-3．569, -4．152,P<0．01)。在PCa中NSBP1的表达水平与PCa病理分期、Gleason评分(GS)、血清前列腺特异抗原(PSA)均不相关(P=1．911,0．666,0．779)。结论NSBP1在PCa中高表达,并可能参与了PCa的发生发展过程。
Objective To investigate the expression of nucleosomal binding protein 1 （NSBP1） in normal prostate （ NP）, benign prostate hyperplasia （ BPH）, and prostate cancer （PCa） and significance thereof. Methods Western blotting was used to detect the expression of NSBP1 in 10 specimens of NP from voluntary donors who died of accidents, and 15 specimens of PCa resected during operation. Immunohistochenmistry was used to detect the NSBP1 expression in 19 specimens of NP resected during complete cystectomy from patients with bladder carcinoma, and 26 specimens of BPH and 40 specimens of PCa, all resected during operation. Results Western blotting showed that the relative optical density （OD） of NSBP1 in the PCa tissue was 0.66 ±0.02, significantly higher than that of the NP tissue （0.26±0.03, t =37. 308, P 〈 0.01 ）. Immunohistochemistry showed that the positive and weak positive rates of protein expression of NSBP1 in the PCa tissue was 80.0% （32/40）, significantly higher than those of the NP tissue （36.8%, 7/19） and BPH （34.6%, 9/26） （t= -3.569 and t= -4. 152, both P〈0.01）. The NSBP1 expression level in the PCa tissue was not correlated with the pathological staging, grade, and serum prostate-specific antigen （P = 0. 911, 0. 666, and 0. 779）. Conclusion Highly expressed in the PCa tissue, NSBP1 protein is may be involved in the carcinogenesis of PCa.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第28期1962-1965,共4页
National Medical Journal of China
基金
国家自然科学基金资助项目(30271295
30371420)
211工程肿瘤学项目基金资助
关键词
前列腺肿瘤
核小体结合蛋白1
免疫组织化学
高速泳动族蛋白
Prostate neoplasms
Nucleosomal binding protein 1
Immunohistochemistry
High mobility group proteins
