摘要
建立了蛋黄磷脂中卵磷脂（即磷脂酰胆碱，PC）的高效液相色谱-蒸发光散射检测（HPLC—ELSD）的测定方法。以Nova-Pak Silica 60A硅胶柱（3．9mmi．d．×150mm，4μm）为分离柱，正己烷-异丙醇-3％冰醋酸水溶液（体积比为35：65：8）为流动相，等度洗脱，流速1．0mL／min，柱温30℃。蒸发光散射检测器漂移管温度50℃，雾化气（空气）压力350kPa。在上述条件下测得PC在0．16—1．61g／L范围内线性关系良好（r^2=0．9979），检测限为0．64μg，方法的精密度为3．2％（n=5），回收率为98．2％～128．2％。将该方法用于实际样品的测定，获得了令人满意的结果。该方法预处理简单，分析速度快，可用于蛋黄磷脂中卵磷脂的测定。
A method for the determination of egg-yolk phosphatidylcholine by normal-phase high performance liquid chromatography （NP-HPLC） with evaporative light scattering detection （ELSD） was developed. Yolk phosphatidylcholine was successfully separated on a Nova-Pak Silica 60A column （3.9 mm i. d. × 150 mm, 4μm）, using hexane-isopropanol-water containing 3% acetic acid （35： 65： 8, v/v/v） as mobile phase at a flow rate of 1.0 mL/min. The drift tube temperature and air carrier gas pressure of the ELSD were set at 50 ℃ and 350 kPa, respectively. The linear detection range was 0.16 - 1.61 g/L （ r^2 = 0. 997 9 ） and the limit of detection （LOD） was 0.64 μg with excellent relative standard deviation （RSD） of 3.2% （ n = 5 ）. The recovery was in the range of 98.2% - 128.2%. The results indicated that this method is accurate, rapid, simple and reproducible, and thus suitable for the analysis of egg-yolk phosphatidylcholine in real samples.
出处
《色谱》
CAS
CSCD
北大核心
2006年第4期373-375,共3页
Chinese Journal of Chromatography
关键词
高效液相色谱法
蒸发光散射检测
磷脂酰胆碱
蛋黄卵磷脂
high performance liquid chromatography （HPLC）
evaporative light scattering detection （ELSD）
phosphatidylcholine （ PC ）
egg-yolk phosphatidylcholine （ EYPC ）
