摘要
目的对在中医理论上具有活血化瘀功效且有抑瘤作用的单味中草药6号和10号药的有效部位T3和M2进行抗血管生成作用研究。方法体外培养人血管内皮EC细胞MTT实验和鸡胚绒毛尿囊膜(CAM)实验。结果T3和M2对人血管内皮EC细胞有明显的抑制增生的作用(P<0.05),抑制率与药物形成较好的浓度梯度关系。CAM实验T3(1g/ml,3μl)作用部位的血管结构破坏,血液溢出,周围血管网减少;M2(4g/ml,3μl)作用部位小血管网减少,对周围的大血管有一定的聚敛、趋化作用。结论有活血化淤功能的中草药6号和10号药的抗癌有效部位T3和M2的抗癌机制之一可能是抗血管生成。
Objective To study the antiangiogenesis effect of active components T3 and M2 from single Chinese herbal medicine No.6 and No.10 with invigorating the circulation of blood in Chinese medicine theory. Methods human vascular endothelial cell （EC） was cultivated in vitro, the MTT assay and CAM experiment were carried out. Results T3 and M2 showed very significant inhibitory effect on EC （P 〈 0.05） and showed very good concentration gradient relationship between inhibitrory rate and the medicine. In CAM experiment of T3, blood vessel struetune was destroyed and blood spillage and peripheral blood vessel decreased, but In M2 test, showed small blood vessel decrease while peripheral big blood vessal gathered and amassed. Conclusion One of the antieaneer mechanisms of active components T3 and M2 of Chinese herbal No.6 and No.10 which stimulating blood eirulation and smoothing away congestion funtion are through antiangiogenesis effect.
出处
《肿瘤研究与临床》
CAS
2006年第7期451-452,455,共3页
Cancer Research and Clinic
基金
内蒙古医学院青年科技项目资助(NY2004QN008)
关键词
活血化瘀
抑瘤作用
中草药
抗血管生成
Antiangiogenesis
Chinese herbal medicine
Invigorating the circulation of blood
