摘要
目的 :研究黄酮类化合物槲皮素对白血病细胞株 NB4的抑制作用。方法 :应用细胞计数、细胞形态、DNA凝胶电泳等方法 ,在体外研究槲皮素诱导 NB4细胞凋亡的作用。结果 :10μmol/ L浓度的槲皮素无明显抑制 NB4增殖作用 ,过高的浓度可能发生细胞变性 ,而当作用浓度为 30μm ol/ L～ 90μmol/ L时 ,就能显示抑制效应 ,其抑制作用呈剂量依赖性 ,细胞表现典型的凋亡特征。结论 :槲皮素在一定的浓度范围内能抑制 NB4细胞增生 ,其作用随时间和浓度增加而增强 ,并诱导凋亡。
Objective:To study the role of quercetin which inhibit the proliferation of leukemia NB 4 cell line.Methods:The effect of quercetin on induction of NB 4 cell line apoptosis was studied by the cell number count,cell morphology,and DNA gel electrophoresis.Rusults:There was no significant difference in cell growth between the concentration of 10μM and control.The higher concentration results in mass death.We verify the results that the quercetin with concentration of (30～90)μmol/L can successfully inhibit the proliferation of NB 4 and the cell still showed apoptosis features.Conclusion:The quercetin with concentration of 30 μmol/L～90 μmol/L could successfully inhibit the proliferation of NB 4 cell lines in a dose ,time dependent manner and inducing apoptosis.
出处
《白血病．淋巴瘤》
CAS
2001年第4期199-201,共3页
Journal of Leukemia & Lymphoma
基金
上海市高教局基金赞助项目 (97B19)
