摘要
目的使用α-氰基丙烯酸烷基酯建立人肝癌-裸鼠皮下肝原位移植瘤模型。方法将BEL-7402人肝癌细胞接种于裸鼠皮下,建立皮下模型。形成皮下移植瘤后再移植于裸鼠肝内,利用α-氰基丙烯酸烷基酯建立肝原位移植瘤模型(间接肝原位移植瘤模型)。结果成功地利用α-氰基丙烯酸烷基酯建立了间接肝原位移植瘤模型。移植瘤存活率达95.8%。结论与传统的肝原位移植瘤模型比较,利用α-氰基丙烯酸烷基酯建立间接肝原位移植瘤模型方法简便,成功率高,便于推广使用。
Objective Using α-cyanoacrylate to establish the orthotopic transplantation tumor model from the subcutaneous model of human hepatocellular carcinomas（HCC）in nude mice. Methods The subcutaneous animal tumor model was established by transplanting BEL-7402 cell into the subcutaneous of nude mice. Then the orthotopic transplantation tumor model was established by injecting the subcutaneous transplanted tumor into liver （indirect orthotopic model）. Results Using α-cyanoacrylate to establish the indirect orthotopic model successfully and the survival rates of the transplantation tumor were 95.8 %. Conclusion It was compared with the traditional methods that the indirect orthotopic transplantation tumor model by using α-cyanoacrylate to establish is an easy method with high successful rate and easy to spread.
出处
《肿瘤研究与临床》
CAS
2006年第7期442-444,共3页
Cancer Research and Clinic
基金
内蒙古自治区教育厅重大项目(ZD9809)
