摘要
报道了玉米低拷贝盐逆境胁迫蛋白基因ｎａｃ１生物素标记的染色体原位杂交结果．供试探针为这个基因的ｃＤＮＡ克隆，其长度仅为５５０ｂｐ．采用酶联免疫级联放大和ＤＡＢ检测系统检测，实验结果表明，杂交信号分布在第２染色体短臂和第１０染色体长臂，与着丝粒的百分距离分别为６７．５６±３．２９１和８０．９２±２．４１１，检出率分别为５．８１％和１０．３２％．
A biotin labeling in situ hybridization technique was first used to physically map a low copy genes nac1 in maize, The cDNA clones of nacl gene which was only 550 bp in size was used as the probe for locating the gene nacl. The cDNA probe was bybridized onto the long arm of the chromosome 10 and the short arm of the chromosome 2. The percent distances from centromere to detection site were 80 92±2.411 and 67.56±3.291 respetively. The detection rates of in situ hybridization were 10.32% and 5.81% respectively. The rseults demonstrated that the gene nac1 had multiple sites in maize genome. The techniques of chromosome preparation; mapping low or single copy genes with ISH in plants have been discussed in this paper.
出处
《华中师范大学学报（自然科学版）》
CSCD
1996年第4期480-485,共6页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金
国家教委博士点基金
关键词
玉米
基因
原位杂交
品种
盐胁迫
逆境胁迫
maize
gene nac1
chromosome in situ hybridizatiom
biotin labeling
