摘要
目的:探讨BTV-HbC3对人肝癌Hep-3B细胞线粒体功能和膜电位的影响及其与细胞凋亡关系。方法:利用透射电镜和荧光显微镜观察Hep-3B细胞感染BTV-HbC3在24,36,48 h的凋亡情况与其线粒体形态变化;噻唑蓝(MTT)法检测其线粒体功能;流式细胞仪检测经Rh123/PI染色的BTV-HbC3诱导该细胞线粒体跨膜电位(△Ψm)和Annexin V/PI染色的细胞凋亡率。结果:Hep-3B细胞感染病毒在24,36,48 h时可导致线粒体功能下降,同时使线粒体膜电位显著降低,细胞凋亡率显著增加。结论:BTV-HbC3通过诱导凋亡来抑制人肝癌细胞的生长,其机制与线粒体跨膜电位下降有关。
Objective： To explore the changes of in human hepatic carcinoma cells Hep-3B after bluetongue virus HbC3 （BTV-HbC3） infection and their relationship with the BTV-HbC3 induced apoptosis. Methods. Morphological changes of apoptosis and mitochondria of Hep-3B cells infected with BTV-HbC3 were observed under fluorescence microscope and transmission electron microscope. Mitochondrial function was assessed with MTT. The mitochondrial transmembrane potential of Hep-3B cells straining Rh123/PI and the apoptosis of Hep-3B cell staining Annexin V/PI were measured by flow cytometry （FCM）. Results： Mitochondrial transmembrane potential and mitochondrial function of Hep-3B cells were significantly decreased at 24, 36 and 48 hours after infection, but the rate of Hep-3B cells apoptosis markedly increased. Conclusion： BTV-HbC3 might inhibit the Hep-3B cells growth by inducing apoptosis and its mechanism might be related to the loss of △Ψm.
出处
《武汉大学学报（医学版）》
CAS
2006年第4期425-428,F0002,共5页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:30271471)
关键词
蓝舌病毒HbC3
细胞凋亡
线粒体
膜电位
人肝癌细胞
Bluetongue Virus HbC3
Apoptosis
Mitochondria
Transmembrane Potential
Human Hepatic Carcinoma Cell
