摘要
目的:探讨大肠癌hMLH1蛋白表达与5-氟尿嘧啶(5-Fu)敏感性的关系。方法:应用流式细胞术测定大肠癌hMLH1蛋白表达,用体外肿瘤药物敏感实验(MTT法)检测大肠癌细胞对5-Fu敏感性,并分析hMLH1蛋白表达与大肠癌细胞对5-Fu敏感性的关系。结果:大肠癌hMLH1蛋白阳性和阴性之间5-Fu敏感性存在显著性差异(χ2=5.97,P<0.05)。结论:临床标本中hMLH1蛋白表达缺失与大肠癌细胞对5-Fu耐药密切相关。
Objective： To investigate the correlation of hMLH1 protein expression and sensitivity of 5- fluorouracil（5-Fu） in colorectal cancer. Methods： Flow cytometry was used to detect the expres- sion of hMLH1 in colorectal cancer and the sensitivity of 5-fluorouracil was measured by MTT test method. Results： The hMLH1 positive cancer was more sensitive to 5-fluorouracil than hMLH1 negative tumor（X^2=5.97, P〈0.05）. Conclusion： A lack of hMLH1 expression may play a role in 5-fluorouracil resistance in colorectal cancer.
出处
《武汉大学学报（医学版）》
CAS
2006年第4期454-455,470,共3页
Medical Journal of Wuhan University
