摘要
目的探讨肿瘤坏死因子-α(TNF-α)基因多态性与广东地区汉族人群散发性阿尔茨海默病(SAD)发病易感性的关系。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,检测44例SAD病人(AD组)及45例正常老年人(对照组)TNF-α基因的TNF-α1及TNF-α2等位基因频率。按比值比(OR)作疾病关联分析。用放免法检测两组的血清TNF-α含量。结果AD组的TNF-α2基因频率为0．182,明显高于对照组的0．078(OR=2．635,P=0．039)；AD组血清TNF-α含量为(567．85±102．43) ng/L,明显高于对照组的(389．73±56．68)ng/L(P＜0．05)。结论广东地区汉族人群中,TNF-α基因多态性与SAD有关联．TNF-α2等位基因与SAD的易感性有关。
Objective To explore the relation between the tumor necrosis factor-α（TNF-α） gene polymorphism and the susceptibility to sporadic Alzheimer＇s disease （SAD） in a Guangdong Han population. Methods The frequencies of TNF-α1 and TNF-α2 alleles of TNF-α gene were detected by polymerase chain reaction-sequence specific primers （PCR-SSP） in 44 SAD cases and 45 normal elders as controls. The differences in polymorphic distribution between the two groups were tested, and odds ratio was computed for association analysis. The content of TNF-α in serum was measured by radioimmunoassay. Results The frequency of TNF-α2 allele was significantly increased in patients with SAD compared to that of the controls （0.182 vs 0.078, OR=2.635, P=0.039）. The content of TNF-α in serum in SAD group was （567.85±102.43） ng/L, which was significantly higher than that （389.73±56.68） ng/L in control group （P〈0.05）. Conclusion In Han population in Guangdong province, TNF-α gene polymorphism is correlated to the risk for SAD. TNF-α2 allele may play a role in SAD susceptibility, while TNF-α1 allele may be a protective gene against SAD.
出处
《中华神经医学杂志》
CAS
CSCD
2006年第7期696-698,共3页
Chinese Journal of Neuromedicine
基金
广东省卫生厅科研基金(E002003015)
