摘要
目的研究TNFα-308基因多态性与甘肃地区乙肝病毒(HBV)感染的关系。方法选择汉族慢性乙型肝炎患者156例,其中男118例,女38例及80例正常对照,将病人分组后采用等位基因特异引物聚合酶链反应(ASPCR)方法,分析甘肃地区TNFα-308启动子基因多态性。结果TNFα-308位各基因型频率在HBV感染者中和在健康对照者中的基因型差异有显著性。TNF1和TNF2等位基因频率两组之间相比差异亦有显著性,且G等位基因与HBV感染有一定关联性。HBV感染者中G/G基因型明显升高。在ALT>50u/L、ALT<40u/L及正常对照组之间,基因型频率差异均有显著性。且A等位基因与ALT的升高有一定的相关性。在对等位基因频率的比较中,应答组与对照组,无应答组与对照组相比差异均有显著性。应答组与无应答组等位基因频率相比差异无显著性。对抗病毒治疗的反应与A等位基因有一定的相关性。HBVDNA定量中高载量组与低载量组TNFα-308位基因型频率相比差异无显著性,而两组与对照组相比差异均有显著性。结论甘肃地区HBV感染者TNFα-308位基因多态性与慢性HBV感染有关。
Objective Some people with HBV infection can develop chronic hepatitis. The reason for viral persistence are still pnorly understood,but host factors are likely to influence the disease outcome. A number of studies showed that the TNF-α system was activated in the liver of patients with chronic HBV infection. We studied the assciation btlwecn patients with HBV in gansu province and the polmorphisms in promoter-region of the TNF-alphe gene at position - 308. Methods We investigated two candidate gene loci in 156 patients with HBV infection and 80 healthl y controls. The polymorphisms were assessed by the ASPCR（ allele-spcific polymerase chain reaction）. Results We found the difference in the TNF-alpha gene at position - 308 genetype distributions between patients with chronic HBV infection and controls. G allele（ OR = 2. 375,95% CI = 1. 612 - 3. 498 ）was associated with the HBV infection. A allele（ TNF-1I/TNF-2 的 OR =0. 687,95% CI =0. 439 - 1. 076）was ,associated with the ALT elevated. A allele （TNF-1/TNF-2 OR = 0. 476, 95% CI = 0. 187 - 1. 212 ） was also associated with the response to antiviral therapy. Conclution The polymorphisms in promoter-region of the TNF-α gene at position-308 was associated with HBV infection.
出处
《临床消化病杂志》
2005年第6期289-292,共4页
Chinese Journal of Clinical Gastroenterology
