摘要
目的探讨多巴胺β羟化酶(DβH)基因编码区589位单核苷酸多态性(SNP)与妊娠期高血压疾病发病的关系。方法采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术,检测107例妊娠期高血压疾病患者(妊娠期高血压疾病组,其中轻度子痫前期39例、重度子痫前期49例、子痫19例)和95例正常晚期妊娠妇女(正常对照组)的DβH基因589位SNP基因型。结果(1)妊娠期高血压疾病组DβH基因589位SNP基因型频率GG、GA、AA分别为75·7%、21·5%、2·8%,正常对照组分别为72·6%、24·2%、3·2%,两组比较,差异无统计学意义(P>0·05)。妊娠期高血压疾病组DβH基因589位SNP等位基因频率G、A分别为86·4%、13·6%,正常对照组分别为84·7%、15·3%,两组比较,差异无统计学意义(P>0·05)。(2)轻、重度子痫前期及子痫孕妇DβH基因589位SNP各基因型频率比较,差异无统计学意义(P>0·05)。结论DβH基因589位SNP与妊娠期高血压疾病发病无相关性,与病情轻重也无相关性。
Objective To investigate the association between the single nucleotide polymorphism （SNP） at locus 589 of dopamine-β-hydroxylase （DβH） gene and hypertensive disorder complicating pregnancy（HDCP）. Methods One hundred and seven pregnant women with hypertensive disorder complicating pregnancy （ HDCP group） and 95 normal pregnant women （ control group） matched for age and gestation were selected. Genotypes of the SNP at locus 589 were typed by polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP）. Results （ 1 ） The frequencies of DβH GG, GA and AA genotypes of the SNP at locus 589 were 75. 7% ,21.5% and 2. 8% in HDCP group, respectively. They were 72. 6%, 24. 2% and 3.2% in normal control group, respectively （ P 〉 0. 05）. The frequencies of the two alleles G and A were 86.4% and 13.6% in HDCP group, and 84. 7% and 15. 3% in normal control group, respectively （ P 〉 0. 05 ）. （2） No evident differences existed in distribution of genotypes of the SNP at locus 589 between mild preeclampsia, severe preeclampsia and eclampsia groups （ P 〉 0. 05 ）. Conclusion The SNP at locus 589 of DβH gene is not associated with hypertensive disorder complicating pregnancy, nor is it associated with the severity of hypertensive disorder complicating pregnancy.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第6期384-386,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
妊娠并发症
心血管
高血压
多巴胺Β羟化酶
多态现象(遗传学)
Pregnancy complications, cardiovascular
Hypertension
Dopamine betahydroxylase
Polymorphism （genetics）
