摘要
目的研究弓形虫韩国KI-1分离株的生物学特性。方法分别将弓形虫韩国KI-1株与国际标准RH株进行下列实验:透射电镜观察虫体的形态,不同浓度的速殖子接种BALB/c小鼠观察其毒力,蛋白质印迹(Westernblotting)分析弓形虫抗原与抗弓形虫单抗Tg563和抗新孢子虫单抗12B4反应,PCR-限制性片段长度多态(PCR-RFLP)技术分析其基因型,检测SAG1和ROP1基因编码区的序列。结果弓形虫韩国KI-1株的形态、毒力与RH株相似。KI-1和RH抗原仅与抗弓形虫单抗Tg563反应,而不与抗新孢子虫单抗12B4反应。PCR-RFLP技术分析表明,KI-1株属基因型I型弓形虫虫株。KI-1株与RH株相比,SAG1和ROP1基因均各有7个核苷酸及6个氨基酸差异。结论KI-1株为基因型I型弓形虫强毒株,与RH株间存在一定的基因差异。
Objective To study the biological characteristics of newly isolated Toxoplasma gondii Korean isolate-1 （KI-1）. Methods The morphology and infectivity of KI-1 were observed by transmission electron microscopy and animal inoculation. Both RH and KI-1 antigens were detected by an anti-T, gondii monoclonal antibody （mAb）, Tg563, and an anti-Neospora caninum mAb, 12B4. The genotype was determined by PCR-RFLP analysis of SAG2 locus. The sequence heterogeneity of the SAGI, ROPI gene coding regions of the RH and KI-1 was detected by an automated sequencer. Results The morphology and infectivity of KI-1 were similar to those of RH strain. Both RH and KI-1 antigens reacted with Tg563, not with 12B4. In comparison to RH strain, the KI-1 showed a difference of seven positions of nueleotide substitutions and six positions of amino acid substitutions in both SAGI and ROPI gene coding regions. Conclusion KI-1 is an isolate of T. gondii with strong virulence. Compared with RH strain, certain genetic differences exist.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第3期183-186,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
韩国科学与工程基金(No.R01-2002-000-00422-0)
韩国教育部BK21人类生命科学基金~~
