摘要
目的：调查上海地区1169名6～15岁学龄儿童细胞色素P450 3A酶活性（cytochrome P450 3A activity，CA）的分布情况及正常值范围。方法：采用HPLC梯度洗脱法测定尿液中氢化可的松和6p羟基氢化可的松的浓度，以地塞米松为内标，采用HP Hypersil ODS柱（250mm×4mm，5μm），流动相A相为CH3CN，B相为7．56mmol／L（NH4）2SO4溶液，梯度设置；0～6．5min，A：10％，B：90％；6．5～15min，A：35％，B：65％，线性变化；15～24min，A：35％，B：65％；24～28min，A：10％，B：90％，线性变化；28～42min，A：10％，B：90％。柱温30℃；流速1．0ml／min,检测波长240nm。数据分布形态检测采用Kolmogorov-Smirnov检验，男、女儿童酶活性之间的比较采用两个独立样本的t检验。结果：1169名学龄儿童CA分布为左偏态分布，偏度系数为2．66，IgCA呈正态分布，偏度系数为0．027。CA正常值范围为1．00～25．47，男女性之间CA无显著性差异（P=0．919）。将未发育期、发育启动期和发育中期分别设为X=1、2、3，与CA平均值（Y）进行线性回归，得到回归方程为Y=0．685X＋3．513（r=0．9999）。结论：确定学龄儿童CA分布情况及其正常值范围，可为制定儿童个体化给药方案和给药剂量提供依据。
Objective： To investigate the distribution of cytochrome P450 3A activity（CA） in the school children aged 6-15 years in Shanghai. Methods： Concentrations of hydrocortisone and 613-hydroxycortisol in urine were determined by gradient HPLC with HP Hypersil ODS column（250 mm×4 mm,5 μm） using dexamethasone as internal standard. The mobile phase A was CH3CN, while mobile phase B was 7.56 mmol/L （NH4）2SO4 water solution. The gradient profile： 0-6.5 min,A： 10% ,B： 90% ;6.5-15 min,A： 35 % ,B：65 % ,linear variation; 15-24 min, A： 35%, B： 65 % ; 24-28 min, A： 10%, B： 90%, linear variation; 28-42 min, A：10 %, B： 90 %. The column temperature was 30 ℃, the flow rate was 1.0 mL/min, and the detection wave length was 240 nm. The frequency distribution of data was examined by the Kolmogorov-Smirnov test. Difference of CA between boys and girls were compared by Student paired t test. Results： The frequency distribution of CA in the 1 169 students was a left skewness distribution with an asymmetry coefficient of 2.66, and IgCA was normally distributed with an asymmetry coefficient of 0. 027. The range of normal values of CA was 1.00-25.47. There was no significant difference of CA between male and female students （P=0. 919）. Pubertal development was graded into stage l（before reaching）, stage 2（just starting） and stage 3 （intermediate）. The linear regression equation between pubertal development （X） and CA（Y） was Y= 0. 685 X ＋ 3.513 （ r = 0. 999 9）. Conclusion： It is useful to determine the distribution of CA and the normal value range of CA in Shanghai school-age children for individual dosage regimen.
出处
《药学服务与研究》
CAS
CSCD
2006年第3期176-178,共3页
Pharmaceutical Care and Research
