摘要
〔目的〕探讨乙型肝炎病毒核酸(HBV-DNA)与HBV血清标志物的关系。〔方法〕将血清样本用荧光定量聚合酶链反应(FQ-PCR)和酶联免疫吸附法(ELISA)分别检测HBV-DNA和乙型肝炎病毒标志物。〔结果〕A组(HBsAg和HBeAg两项阳性)、B组(大三阳)、C组(HBsAg、HBcAb两项阳性)和D组(小三阳)的HBV-DNA阳性率分别为97.06%、88.24%、57.14%和43.33%,HBV-DNA量的对数均值分别为6.0267、5.5667、4.9639、5.1520、5.4574,各组间HBV-DNA阳性率和HBV-DNA量均存在显著性差异(P<0.05);178份HBV-DNA阳性标本中HBsAg100.00%阳性;HBsAg阳性、HBeAg阳性和HBsAg阳性,HBeAg阴性组中HBV-DNA阳性率分别为89.34%和44.52%,存在显著性差异(P<0.05)。〔结论〕HBsAg和HBeAg两个阳性组病毒复制最活跃,HBV-DNA阳性率和HBV-DNA量显著高于其它组别;HBeAg与HBV-DNA密切相关,但不能完全反映HBV在体内的复制情况,将FQ-PCR定量检测作为血清学方法的补充具有重要的临床意义。
Objective To investigate the relationship between HBV DNA and HBV serum markers.Methods HBV-DNA was determined by FQ-PCR and serum markers were tested by ELISA. Results The positive rate were 97.06%～88.24% ～57.14% and 43.33% respectively, for HBV-DNA in group A （HBsAg, HBeAg positive）,group B （HBsAg,HBeAg and HBcAb positive）, group C （HBsAg and HBeAg positive ） and group D （HBsAg, HBeAb and HBcAb positive） .The HBV-DNA denary logarithm value were 6.0267 （SD=1.6279）、5.5667 （SD=1.5806）、4.9639（SD=1.4729）、5.1520（SD=1.4331）、5.4574（SD=1.5709）in group A, group B, group C and group D respectively. There was significant difference for HBV-DNA positive rate and HBV-DNA values among these groups （P〈 0.05） .All of 178 samples,HBV-DNA positive were 89.34% and 44.52% in group of HBsAg（＋）、HBeAg（＋） and group of HBsAg（＋）, HBeAg（-） respectively. There was a significant difference between these two groups. Conclusions The HBV DNA positive rate and HBV-DNA Value are significantly higher than any other group, HBeAg correlated strongly with HBV-DNA, but it can＇t absolutely reflecting HBV infection. FQ-PCR has the important clinical value as a supplement to serum detection methods.
出处
《中国国境卫生检疫杂志》
CAS
2006年第3期143-145,共3页
Chinese Journal of Frontier Health and Quarantine
关键词
聚合酶链反应
荧光定量PCR
肝炎病毒
乙型
血清
标志物
Fluorescence quantitative polymerase chain reaction（FQ-PCR）
Hepatitis B virus
Serum
Markers
