摘要
目的探讨芦荟多糖(AloePolysaccharides,AP)对体外培养的小鼠腹腔巨噬细胞一氧化氮(nitricox-ide,NO)生成和诱导型一氧化氮合酶(induciblenitricoxidesynthase,iNOS)酶活性的影响。方法体外培养小鼠腹腔巨噬细胞,分别用0～400mg/L浓度的AP刺激24h,采用Griess反应测定NO生成量,荧光法检测iNOS活性。结果AP在25～400mg/L浓度范围内作用于小鼠腹腔巨噬细胞24h后,NO生成量增加、iNOS活性增高,除低浓度AP组(25mg/L)外,其余各浓度组差别均具有非常显著性意义(P<0.01),并呈显著正相关(r=0.7523,P<0.01);转录抑制剂放线菌素D、蛋白质合成抑制剂放线菌酮和NOS竞争性抑制剂L-NMA,均能有效抑制AP所诱导的巨噬细胞NO生成和细胞内iNOS活性(P<0.01)。结论AP能促进细胞内iNOS基因表达,以从头合成方式促进细胞NO合成和释放,所释放的NO可能参与AP的免疫调节过程。
Objective To investigate the effects of nitric oxide and inducible nitric oxide synthase （iNOS） activity of mouse peritoneal macmphages in vitro by Aloe Polysaccharides. Methods The mouse peritoneal macmphages were stimulated in 24h with 0-400mg/L concentration respectively. NO production and iNOS activity were determined by Griess and fluorometric method. Results Stimulated mouse peritoneal macrophages were with 25-400mg/L concentration respectively after 24 h, NO production and iNOS activity was increased, and there were significant differences in all groups（P〈0.01） except low AP concentrafion（25mg/L） and notable positive correlation（r=0.7523,P〈0.01）. The result also showed that mRNA transcriptional inhibitor Actinomycin D, protein synthesis inhibitor Actidione and NOS competitive inhibitor N-monomethyl-L-arginine（L-NMMA） could effectively inhibit NO production and intracellular iNOS activity of macrophages induced by AP （P〈0.01）. Conclusion AP can enhance intracellular iNOS gene expression, synthesis and releasing of cellular NO. NO may take part in the immunoregulation of AP.
出处
《湖南中医学院学报》
2006年第3期20-22,共3页
Journal of Hunan College of Traditional Chinese Medicine
基金
广东省重点学科资助课题(200308)。
