摘要
建立西藏产舌岩白菜中岩白菜素含量的反相高效液相色谱分析方法。色谱柱：ZORBAX SB-C18柱（250mm×4．6mm，5μm）；色谱条件：检测波长274nm，柱温35℃，流速0．8ml／min；流动相：甲醇：水=25：75。利用正交试验确定了超声提取的最佳条件，结果表明，在选定条件下岩白菜素与其他组份达到了基线分离，岩白菜素的线性范围为0．306—2．14μg，r=0．9998，回收率为101％（RSD=2．2％），该方法快速、简便。结果准确。
Objective： To establish a quantitative method of detemination of bergenin in Bergenrae pacumbis by RP-HPLC. Method： The sample were separated on the column of Zorbax SB-C18（2.50 mm×x 4.6mm, 5 μm） which eluted with methanol and water（2.5：75）. Detected wavelength at 274 rim, flow rate at0.8 ml/min, column temperature at 35 ℃; The Supersonic extraction of bexgenin was studied by orthogonal experiments. Results： Bergenin was base-isolated. The linear ranges of bergenin was 0.306-2.14 tag（ r = 0.999 8） ; The average recoveries were 101% （ RSD = 2.2% ）. Conclusions： The method is rapid and precise.
出处
《安徽农业科学》
CAS
北大核心
2006年第11期2323-2324,共2页
Journal of Anhui Agricultural Sciences
