摘要
为评价2种常用免疫抑制方法对小白鼠排人源隐孢子虫卵囊规律的影响,将19日龄小白鼠随机分成2组,第1组通过饮水给予地塞米松,第2组采用导胃管灌服地塞米松。第7 d,2组小白鼠灌胃接种人源隐孢子虫,每只小白鼠感染量为1.5×106个卵囊。两组均在感染当天就有卵囊排出,感染后第6 d粪便卵囊计数明显增加,随之出现3个高峰期,此后逐渐下降。免疫抑制组小白鼠在卵囊持续期不断有死亡但无腹泻症状。试验结果表明,用这2种免疫抑制方法,均能成功感染人源隐孢子虫,但通过灌服地塞米松可使小白鼠获得更高的OPG(每克粪便中的卵囊数)值和更长的排卵囊持续期。
To estimate the affection of the two common immunosuppression methods on discharging oocysts rule. 19 days old mice were divided into two groups randomly, and then different immunosuppression methods were adopted by this experiment. The first group was given the dexamethasone （DEX） by drinking water,while the second group were given intragastrically with pipe,after 3 days of inoculated with cryptosporidium oocysts （CSO）,each mouse was inoculated with 1×10^6 CSO. The mice discharged the oocysts in the first day after inoculated,and in the DIP6, the counted number of the CSO increased obviously, 3 peaks of occysts discharging appeared,and then dropped gradually,during this period,some of the mice died, but no diarrhea symptom was discovered . Comparing with the two groups, it was concluded that the number of the oocysts discharged by the second group was larger than that of the first one, and so did the persistence period of the peak. After using both of the two immunosupprssion methods, we can establish the animal model successfully when inoculated by CSO, and the efficacity of the second method is better than the first one.
出处
《中国畜牧兽医》
CAS
2006年第6期56-59,共4页
China Animal Husbandry & Veterinary Medicine
基金
河南省高校杰出科研人才创新工程项目(2004KYCX002)
关键词
隐孢子虫
小白鼠
免疫抑制
动物模型
cryptosporidium
mouse
immunosuppression
animal model
