摘要
目的为了解林州地区儿童肠道寄生虫的感染情况,尤其是机会性致病原虫和人兽共患寄生虫的流行情况。方法2007年10月至2008年5月,对林州地区8个乡镇进行了调查。采用卢戈氏碘液染色法、饱和蔗糖溶液漂浮法、改良抗酸染色法和分子生物学方法对林州地区23个调查点的1949名15岁以下儿童进行了检测。结果寄生虫总感染率为1.33%(26/1949),共查出6种肠道寄生虫,其中原虫和蠕虫均为3种,感染率分别为0.72%和0.62%。男女寄生虫感染率分别为1.27%(14/1103)和1.42%(12/846),差异无统计学意义(P>0.05)。姚村镇、城郊乡、茶店乡、临淇镇、五龙镇、东姚镇、横水镇和采桑镇儿童感染率分别为1.76%、1.42%、0.83%、2.71%、0.00%、0.74%、1.45%、0.75%和1.33%,各个乡镇之间感染率差异无统计学意义(P>0.05)。首次摸清了林州地区儿童隐孢子虫感染的流行状况,感染率为0.15%。对分离到的1例等孢球虫进行了分子鉴定,序列分析结果显示与雀类的一种球虫Atoxoplasma sp.(AY331571)最为接近,同源性达99.4%,表明可能为鸟类粪便污染所致。结论林州地区儿童肠道寄生虫感染率较低,但仍应进一步开展健康教育,普及卫生知识,提高自我保健意识和自我保健能力,预防寄生虫病的发生和流行。
Objective To understand the prevalence of intestinal parasitic infection in children in Linzhou,especially the epidemiological status of opportunistic pathogenicity of protozoa and zoonotic parasites.Methods An investigation was conducted in eight towns in Linzhou from October 2007 to May 2008.A total of 1949 fecal samples from younger than 15 years old children from 23 sampling points were examined using the Sheather s sugar flotation,modified acid-fast stain and Lugol s iodine-solution stain methods.Results The overall infection rate was 1.33% （26/1949）. Six intestinal parasites were identified, 3 belong to protozoa and 3 belong to venues with infection rates of 0.72% and 0.62% ,respectively. The infection rates in male and female children were respectively 1.27% （14/ 1103） and 1.42% （12/846）, with no significant difference（P〉0.05）. The infection rates of Yaoeun, Chengjiao, Chadian,Linqi,Wulong,Dongyao, Hengshui, and Caisang towns were 1.76%, 1.42%, 0.83%, 2.71%, 0.00%, 0.74%,1.45% ,0.75% ,and 1.33% ,respectively and without any significant difference. This is the first report of Cryptosporidium spp. infection in Linzhou, with the infection rate of 0.15%. Moreover, an lsospora sp. isolate was identified by molecular method. The sequence analysis showed that the isolate was close to Atoxoplasma sp. （AY331571） previously reported in a sparrow, with the identity of 99.4%. The results indicated that it was possibly contaminated by the feces from birds. Conclusion The infection rate of intestinal parasites was low in children in Linzhou, however, further health educations are strongly needed to improve the health knowledge, health concern and health protection of the people in order to reduce the prevalence of parasitic disease.
出处
《热带医学杂志》
CAS
2009年第10期1184-1187,共4页
Journal of Tropical Medicine
基金
国家科技部自然资源平台项目(No.2005DKA21100)
关键词
儿童
肠道寄生虫
感染
林州
children
intestinal parasites
infection
Linzhou
