摘要
用电化学方法对茜素络合剂-镨（Ⅲ）二元络合物与牛血清白蛋白（BSA）的相互作用进行了研究，发现在pH4．9的六次甲基四胺[（CH2）5N4]缓冲液中，茜素络合剂-镨（Ⅲ）络合物能与牛血清白蛋白生成一种非电活性的络合物，导致茜素络合剂-镨（Ⅲ）的峰电流降低，峰电流降低值△ip在一定范围内与BSA的浓度呈线性关系，线性范围为1．5～17．5mg·L^-1，相关系数为0．997，检出限为1．5mg·L^-1，RSD为2．1％，回收率为102％，该法可用于蛋白质的测定。
An electrochemical study on the interaction of the binary complex alizarine-complexan （ALC）- Pr（ Ⅲ ） with bovine serum albumin （BSA） was reported in this paper. In a medium of urotropine buffer （pH 4. 9）, Alizarine complexan-Pr（ Ⅲ ） reacts with BSA to form an electrochemically non-active complex, leading to a deerease in peak current of Alizarine complexarrPr （Ⅲ）. The decrease, of peak current Δip is proportional to BSA concentration in the range of 1.5-17. 5 mg · L^-1 with correlation coefficient of 0. 997. Detection limit of 1.5 mg · L^-1 , RSD of 2. 1% and recovery of 102% were found. The method has been applied to the determination of BSA.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2006年第6期437-439,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
