摘要
目的:研究A型肉毒毒素(botulinum toxin A, BTA)腹腔注射(ip)对大鼠小肠通过速度及胆碱酯酶(ACHE)和P物质(substance P,SP)表达的影响．方法:8周龄Wistar♂大鼠24只随机分为A,B, C,D4组,每组6只;A,B,C组分别ip含BTA 4, 2,1 U的生理盐水2 mL,D组为对照组,ip生理盐水2 mL．4 wk后行小肠碳沫推进实验,取回肠末段标本行免疫组织化学染色对比AChE及 SP的表达．结果:A,B组大鼠的小肠碳沫推进百分比(分别为53．9％±4．0％和53．2％±3．0％)小于D组 (66．4％±5．6％;P<0．05),但C组(64．3％±5．6％) 与D组无统计学差异;A,B组AChE和SP在回肠肌层及黏膜层表达减少,整合光密度与D组相比差异显著(肌层ChE:99．3±39．3,76．3± 36．4 vs 260．1±114．8,P<0．05;黏膜层AChE: 224．6±85．4,230．1±120．7 vs 230．1±120．7, P<0．05;肌层SP:161．2±90．6,200．7±65．5 vs 355．9±70．9,P<0．05;黏膜层SP:198．6±60.3, 229．7±71．8 vs 412．5±79．2,P<0．05),C组与D 组无统计学差别(P>0．05)．结论:BTA ip能减慢大鼠的小肠碳沫推进速度,减少回肠末段AChE和SP的表达．
AIM： To investigate the effects on small intestinal passage velocity and expression of acetylcholinesterase （ACHE） and substance P in rats by injection of botulinum toxin A （BTA） intraperitoneally.
METHODS： Twenty-four Wistar rats of 8 weeks were randomly and averagely divided into 4 groups. The rats in group A, B and C were intraperitoneally injected with 4, 2, and 1 U BTA containing in 2 mL normal saline, while those in group D were treatment with the same amount of normal saline （2 mL）. Charcoal suspension pushing test of small intestine and immunohistochemistry of AChE and Substance P in the end ileum were performed 4 wk after injection. RESULTS： The propelling rate of charcoal suspension at the end of 4 weeks in small intestine was significantly lower in group A （53.9% ± 4.0%）and B （53.2% ± 3.0%） in comparison with that in group D （66.4% ± 5.6%）（P 〈 0.05）. There was no marked difference between group C （64.3% ± 5.6%） and D. The expression of AChE and SP were decreased after injection and the integrated optical density were notably lower in group A and B than those in group D （muscular ACHE： 99.3 ± 39.3, 76.3 ± 36.4 vs 260.1 ± 114.8, P 〈 0.05; mucosal ACHE： 224.6 ± 85.4, 230.1 ± 120.7 vs 230.1 ± 120.7, P 〈 0.05; muscular SP： 161.2 ± 90.6, 200.7 ± 65.5 vs 355.9 ± 70.9, P 〈 0.05; mucosal SP： 198.6 ± 60.3, 229.7 ± 71.8 vs 412.5 ± 79.2, P 〈 0.05）. There were no significant differences between group C and D （P 〉 0.05）.
CONCLUSION： Intraperitoneal injection of BTA can decrease the passage velocity in small intestine and the expression of AChE and SP in rats.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第14期1412-1415,共4页
World Chinese Journal of Digestology
关键词
A型肉毒毒素
小肠通过速度
胆碱酯酶
P物质
大鼠
Botulinum Toxin A
Small Intestinal Passage Velocity
Acetylcholinesterase
Substance P
Rats
