摘要
目的探讨乳腺癌组织中缺氧诱导因子-1α(hypoxia-induciblefactor1alpha,HIF-1α)的表达及其与增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)及临床病理因素的关系。方法采用免疫组化SP法检测乳腺纤维腺瘤、普通乳腺增生组织和乳腺癌中HIF-1α、PCNA蛋白的表达。结果HIF-1α蛋白在乳腺纤维腺瘤、普通乳腺增生组织中不表达;乳腺导管内原位癌中阳性率55%(11/20),浸润性乳腺癌中85%(51/60);HIF-1α在淋巴结、雌激素受体状态及组织学分级中表达有显著差异。乳腺癌中PCNA阳性率75%(60/80),其中原位癌为65%(13/20),浸润癌为78.3%(47/60)。乳腺癌中HIF-1α表达与PCNA显著正相关(r=0.693,P<0.01)。结论HIF-1α在乳腺癌组织中明显上调,与肿瘤细胞增殖、淋巴结转移、雌激素受体状态及组织学分级相关,提示HIF-1α对乳腺癌的肿瘤细胞增殖、生长和侵袭转移起重要作用,有望成为肿瘤治疗的新靶点。
Objective To investigate the expression of hypoxia-inducible-1 alpha（HIF-1α） in human breast cancer and its relationship to proliferating cell nuclear antigen（PCNA） protein, other tumor biomarkers and clinical pathologic factors. Methods Imunohistochemical staining（SP） was used to measure the expression of HIF-1α, PCNA in human breast fibroadenoma, usual hyperplasia and breast carcinoma. Resuits HIF-1α was not found expressing in breast fibroadenoma and hyperplastic lesions. In contrast, the positive rate of HIF-1αwas found in the ductal carcinoma in situ 55%（DCIS, 11/20） and the invasive breast carcinoma 85% （51/60）. The total positive rate of PCNA in breast carcinoma was 75% （60/80）, that in DCIS was 65%（13/20） and that in invasive carcinoma was 78. 3（47/60）. The increased levels of HIF-1α were statistically positively related to that of PCNA（r= 0. 693,P〈0. 01）. Conclusion The expression of HIF-1α was upregulated in breast carcinoma, and HIF-1α was associated with tumor cell proliferation, lymph node metastasis, ER status and stages of histology. These suggest that it will play an important role in the tumor cell proliferation, progression, metastasis of breast carcinoma. It was wished to become a new target for tumor treatment.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第6期391-393,共3页
Cancer Research on Prevention and Treatment
关键词
乳腺癌
缺氧诱导因子-1Α
肿瘤细胞增殖
Breast carcinoma
Hypoxia-inducible factor 1 alpha （HIF-1α ）
Tumor cell proliferation
