摘要
在生物催化法生产对苯二甲酸（PTA）菌种的筛选过程中，建立了高效液相色谱法测定某些假单胞菌的发酵液中对苯二甲酸及中间产物对羧基苯甲醛（4-CBA）、对甲基苯甲酸（p-TOL）的分析方法。优化了色谱条件，在6min之内完全分离PTA及与其结构类似的4-CBA、p-TOL。本法测定PTA的线性范围为0．2～200μg／mL，线性相关系数为0．9998，相对标准偏差为1．52％。
A high performance liquid chromatography method for separation and determination of terephthalic acid in fermentation broth was described. In this work, terephthalic acid （PTA）, 4-carboxybenzaldehyde（4-CBA） ,and p-toluic acid（p-TOL） were completely separated and determined in 6 minutes. It was proved to be a rapid, effective way to determine and control the PTA in fermentation broth. Linear range of the method was 0.2～200μg/mL. The relative standard deviations was 1.52%.
出处
《分析科学学报》
CAS
CSCD
2006年第3期333-335,共3页
Journal of Analytical Science
关键词
对苯二甲酸
高效液相色谱
菌种筛选
生物催化
Terephthalic acid
High performance liquid chromatography
Screening strain
Biocatalysis
