摘要
目的研究氯化锂对人SMMC-7721肝癌细胞增殖和对荷肝癌小鼠肿瘤生长的抑制作用。方法采用噻唑蓝(MTT)法观察氯化锂(LiCl)对SMMC-7721细胞增殖的抑制作用;以流式细胞仪分析氯化锂处理SMMC-7721肝癌细胞后,细胞凋亡情况;同时利用小鼠H22肝癌细胞实体瘤模型,观察氯化锂对荷肝癌小鼠肿瘤生长的抑制作用;对肿瘤组织超微结构进行透射电镜观察。结果氯化锂对SMMC-7721细胞具有抑制作用并有浓度依赖性;且氯化锂可诱导SMMC-7721细胞发生凋亡;荷肝癌小鼠动物实验中氯化锂中浓度组和高浓度组抑瘤率分别达到50.0%和60.7%,提示氯化锂对小鼠的肿瘤生长具有一定的抑制作用;电镜下可见氯化锂处理组肿瘤组织的凋亡细胞和凋亡小体。结论氯化锂对SMMC-7721细胞的增殖和荷肝癌小鼠肿瘤的生长具有抑制作用,作用机制可能与其诱导肝癌细胞凋亡有关。
Objective To study the effects of Lithium chloride（LiC1） on inhibition of the proliferation of human SMMC-7721 cells and the growth of Tumor in hepatocarcinoma-bearing mice. Methods Using MTT assay to detect antiproliferation effect of Lithium chloride on SMMC-7721 cells, applying Flow cytometry（FCM） to observe whether Lithium chloride can induce apoptosis in SMMC-7721 cells or not; mouse hepatocellular carcinoma cells H-were subcutaneously injected into KM mice to establish animal model of hepatocarcinoma-bearing mice,and LiCl were administered to the tumor-bearing mice;the kinetics of tumor formation and tumor growth were measured, and tumor growth inhibition rate was calculated;Tumor tissue samples were taken and examined to assess the inhibitory effect of LiCl on tumor growth in the mice. Results LiCl could significantly prevent the proliferation of SMMC-7721 cells and showed concentration dependency. It could also induce apoptosis in SMMC-7721 cells; Marked inhibitory effect of LiCl on transplanted hepatocellular carcinoma H22 was observed in the tumor-bearing mice, the inhibitory rates were 50.0% and 60.7% in the groups treated with middle and high dosage of LiCl. Apoptotic cells and apoptotic bodies were observed in the groups treated by LiCl under the electron microscope. Conclusion LiCl had marked inhibitory effect on the proliferation of SMMC-7721 cells and the tumor growth of hepatocarcinoma-bearing mice which was probably related to induction of apoptosis.
出处
《实验动物与比较医学》
CAS
2006年第2期83-87,104,共6页
Laboratory Animal and Comparative Medicine
