摘要
目的检测外周血CD4+及CD8+T淋巴细胞表面CD81、CCR5的表达,探讨与丙型肝炎病毒(HCV)单纯感染、人类免疫缺陷病毒(HIV)单纯感染和HCV/HIV合并感染的关系。方法采用流式细胞术,检测HCV感染组(n=21)、HIV感染组(n=14)、HCV/HIV感染组(n=28)及正常对照组(n=30)人外周血CD4+及CD8+T淋巴细胞表面CD81和CCR5的表达。结果与正常对照组相比,外周血CD4+T淋巴细胞表面CD81,在HCV感染组表达变化不明显,而在HIV感染组和HCV/HIV合并感染组中低表达;CD8+T淋巴细胞表面CD81,在HCV感染组、HIV感染组和HCV/HIV合并感染组中都呈高表达。外周血CD4+T和CD8+T淋巴细胞表面CCR5,在HIV感染组高表达,而在HCV感染组和HCV/HIV合并感染组中低表达。结论HCV/HIV合并感染中,影响外周血CD4+及CD8+T淋巴细胞表面CCR5表达的主要因素是HCV感染;而合并感染对CD4+及CD8+T淋巴细胞表面CD81的表达,其影响作用是不相同的。
Objective To investigate the expression of CD81 and CCR5 on CD4 ＋/CD8 ＋ T lymphocytes in the peripheral blood of patients with HCV mono-infection, HIV mono-infection and HCV/ HIV co-infection as well as the correlation between them. Methods We measured the expression of CD81 and CCR5 on CD4＋/CD8 ＋ T lymphoeytes in peripheral blood samples from 21 patients with HCV mono- infection, 14 patients with HIV mono-infection, 28 patients with HCV/HIV co-infection and 30 healthy volunteers by FACS. Results The expression of CD81 on CD4 ＋ T lymphoeytes in HCV mono-infection was not different from healthy control, but decreased significantly in HIV mono-infection and HCV/HIV co- infection. The expression of CD81 on CD8 ＋ T lymphoeytes increased significantly in HCV mono-infection, HIV mono-infection and HCV/HIV co-infection. The expression of CCR5 on CD4 ＋ and CD8 ＋ T lymphocytes increased significantly in HIV mono-infection, but decreased significantly in both HCV mono- infection and HCV/HIV co-infection. Conclusion In HCV/HIV co-infection, HCV may have effect on the expression of CCR5 on CIM ＋ and CD8 ＋ T lymphocytes, while, HCV/HIV co-infection may have different ,effect on the expression of CD81 an CD4 ＋ and CD8 ＋ T lymphocytes from poripheral blood.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第5期398-401,共4页
Chinese Journal of Laboratory Medicine
基金
国家"十五"科技攻关课题(2004BA719A12)
辽宁省教育厅高等学校科学研究项目(2004C049)
