摘要
目的研究肿瘤细胞总唾液酸及α2,6-连接唾液酸对乳腺癌细胞MDA-MB435黏附力的影响。方法乳腺癌细胞MDA-MB-435转染顺义或反义α2,6唾液酸转移酶(ST6Gal-I)cDNA,以黑接骨木凝集素SNA筛选高、低表达α2,6唾液酸细胞克隆,检测各转染细胞的同源凝集作用,并比较唾液酸酶处理前后肿瘤细胞对胶原IV黏附力的变化。结果顺义转染克隆细胞-细胞之间的同源黏附能力下降,与胶原IV的黏附增强;反义转染克隆细胞-细胞之间同源黏附能力增强,而与胶原IV的黏附降低。唾液酸酶处理后肿瘤细胞与胶原IV的黏附力下降至未处理前的31%￣57%。结论乳腺癌MDA-MB435细胞表面唾液酸及α-2,6唾液酸化对肿瘤细胞的黏附有重要影响。
Objective To investigate the effect of cell surface sialic acid and its linkage on the cell-cell and cell-matrix adhesion of mammary carcinoma cells MD-MB-435. Methods MD-MB-435 cells were sense-transfected with ST6Gal I cDNA or antisense-transfected with part of the ST6Gal I sequence inserted in pcDNA 3.1 vector, with mock transfection with pcDNA3.1 vector as the control. The cell surface α2, 6-linked sialylation was determined by fluorescence-activated cell sorting （FACS） using lectin SNA （Sambucus nigra agglutinin specific to α2, 6-linked sialic acid on N-linked glycoprotein）. A significantly increased α2, 6-sialylation subclone in sense-tranfectants and a decreased α2, 6-sialylation subclone in antisense-transfectants were selected for further examination of cell-cell and cell-matrix （collagen Ⅳ） adhesion. The transfectants were also treated with sialidase to compare the capacity of cell adhesion affected by cell surface sialylation. Results Sense-transfection subclone showed a reduced cell-cell aggregation but enhanced cell-matrix adhesion. In contrast, the antisense-transfection subclone exhibited increased cell-cell aggregation and decreased cell-matrix adhesion. After treatment with sialidase, the cell-matrix adhesion of all the transfectants and the parental MDA-MB-435 cells were significantly reduced to the level of 31%-57% of untreated cells. Conclusion Cell surface sialie acid and α2, 6-linked sialylation play an important role in cell-cell and cell-matrix adhesion of mammary carcinoma cell MDA-MB-435.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第6期742-746,共5页
Journal of Southern Medical University
基金
暨南大学引进优秀人才科研启动基金(51204020)
教育部留学回国人员科研启动基金(2004-527)
国家973项目子课题(2001CB510208)
