摘要
目的:调查蒙、汉族人群小肠脂肪酸结合蛋白(IFABP)基因exonⅡ54位点编码丙氨酸或苏氨酸(A/T)单核苷酸多态性(SNP),探讨不同种族、饮食习惯与IFABP基因多态性频率分布的关系.方法:采用聚合酶链反应(PCR),DNA限制性内切酶酶切及基因测序等技术,分别对208例牧区蒙古族人群、150例张家口市区蒙古族人群和190例汉族人群54A/TIFABP基因型分析.结果:牧区蒙古人群54T等位基因频率为0.51,54A等位基因频率为0.49;市区蒙古族人群54T等位基因频率为0.33,54A等位基因频率为0.67;汉族人群54T等位基因频率为0.30,54A等位基因频率为0.70.与市区蒙古族人群、汉族人群相比,牧区蒙古族人群突变型54T等位基因频率明显增高,且差别有统计学意义(分别为χ2=22.98,P<0.01;χ2=34.23,P<0.01).市区蒙古族和汉族相比较,突变型54T等位基因频率无明显差异(χ2=0.47P>0.05).结论:蒙、汉族人群IFABP基因54A/T多态性频率分布无种族差别;牧区蒙古族人群突变型54TIFABP基因高频率分布可能与高脂饮食习惯有关.
AIM： To investigate the single nucleotide polymorphism （SNP） at the 54Ala/Thr （A/T） in the intestinal fatty acid binding protein （IFABP） gene in Hans and Mongolians. METHODS： Polymerase chain reaction （PCR）, restriction endonuclease （HhaⅠ） digestion and DNA sequencing technique were performed to detect the IFABP gene polymorphism at the 54Ala/Thr in 208 Mongolians of pastoral area, 150 Mongolians of Zhangjakou city and 190 Hans. RESULTS： The allelic frequency of 54Thr was 0.51, 0.33, and 0.30, while that of 54Ala was 0.49, 0.67, and 0.70 in Mongolians of pastoral area,Zhangjakou city and 190 Hans, respectively. In comparison with that of Mongolians in urban area and Hans, the allelic frequency of codon 54Thr in Mongolians of pastoral area was sig- nificantly increased （x^2 = 22.98, P 〈 0.01; x^2 = 34.23, P 〈 0.01, respectively）, however, it was not significantly different between the Mongolians of urban area and Hans. CONCLUSION： The IFABP gene polymorphism at 54A/T has no correlation with ethnics among Mongolians and Hans, and the high frequency of 54Thr mutant genotype in Mongolians of pastoral area may be associated with high-fat dieting.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第13期1309-1313,共5页
World Chinese Journal of Digestology
