摘要
目的:观察加味止嗽散(MZ)、MZ脂溶性部位群(MZC)和水溶性部位群(MZS)对过敏性哮喘豚鼠血液和支气管肺泡灌洗液(BALF)中嗜酸粒细胞(EOS)、内皮素-1(ET-1)、一氧化氮(NO)含量及对肺组织结构的影响。方法:采用Wright染色法计血液和BALF中的EOS。采用放射免疫法和硝酸还原酶法分别测定哮喘豚鼠血清和BALF中ET-1、NO的含量。光镜和电镜下观察豚鼠肺组织的结构变化。结果:哮喘模型组豚鼠体内的EOS、ET-1、NO的量明显高于正常对照组(P<0．01),肺组织病理学和超微结构发生明显改变。与哮喘模型组比较,MZ和MZC治疗组能降低豚鼠血液和BALF中EOS、ET-1、NO的含量(P<0．01或P<0．01)和减轻肺组织病理改变,MZS组则未见有明显的作用。结论:MZ中具有平喘作用的物质大多数集中在MZC中,其平喘作用机制可能与其能减少哮喘豚鼠体内EOS数量、恢复ET-1、NO平衡和减轻肺组织结构损伤有关。
Objective ： To study the effects of different fractions of Modified Zhisousan （ MZ, MZC, MZS） on the contents of nitric oxide （NO）, endothelin-1 （ET-1） and eosinophilia （EOS） in the allergic asthma guinea pigs and observe the pathology changes of lung tissue, Methods： The number of EOS in the blood and bronchialveolar lavage fluid （BALF） was counted by Wright staining. The contents of ET-I and No in serum and BALF were analyzed by RIA and nitric acid reductase method. The guinea pig lungs were ob- served under the optical and electron microscope. Results： The number of EOS and the contents of ET-1 and NO in model group were higher than those in control group （ P 〈 0.01 ）. The pathological changes of lung were obvious. The number of EOS and the contents of ET-1 and NO were descended remarkably （ P 〈 0.05 or P 〈 0. 01 ） and the pathological changes in the lung tissue were lightened obviously in MZ and MZC groups, but MZS group had no such effects. Conclusion ： MZC is the effective part of MZ and the anti-asthmatic mechanisms ware related to its significant reduction in contents of ET-1, NO, EOS and the damage of lung tissue possibly.
出处
《中药材》
CAS
CSCD
北大核心
2006年第5期462-464,共3页
Journal of Chinese Medicinal Materials
