摘要
目的探讨人工合成单体抗氧化剂氨基胍(2-AG)对人胚肺二倍体成纤维细胞(2BS)复制性衰老的影响及其分子机制。方法观察2-AG 对2BS 细胞衰老表型、细胞代龄、传代速度、细胞周期、细胞增殖能力、晚期糖基化终末产物(AGEs)水平及衰老相关β半乳糖苷酶(SA-β-GaD阳性率的影响。结果 2-AG 可维持细胞的非衰老表型,增加2BS 细胞代龄19～20代,MTT 法分析发现用2-AG 孵育细胞84h,细胞增殖能力较对照组升高36%～40%。2-AG 显著加快了细胞的增殖速度,其培养细胞 S 期的比例较对照细胞升高约1倍。另外,2-AG 连续培养的细胞在老龄阶段 AGEs、SA-β-Gal 阳性率均显著低于老年对照细胞,而与年轻2BS 细胞相似。结论 2-AG 可有效延缓2BS细胞的复制性衰老。
Objective To study the effect of aminoguanidine （2-AG） on the replicative senescence of human diploid fibroblasts （2BS）. Methods The changes of senescent phenotype, passages, cell cycle, the potential of cell proliferation, the levels of advanced glycation end products （AGEs） and the positive cell rates of senescence-associated β-galactosidase （SA-β-Gal） of AG-effected 2BS cells were detected and analyzed. Results 2-AG maintained unaltered cell morphology and increased life span of 2BS by at least 19-20 passages. The proliferation potential of 2BS cells incubated with 2-AG for 84 h was increased by 36%-40%. The percentage of the S-phase cells cultured by 2-AG was the double of that in the control cells. In addition, the levels of AGEs and the SA-β-Gal positive cell rates were significantly lower in late passage cells in sequenced multigeneration culture in 2-AG medium than those of late passage control cells, and similar to those of young cells. Conclusions 2-AG delays the replicative senescence of 2BS cells.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2006年第5期367-370,共4页
Chinese Journal of Geriatrics
基金
国家重点基础研究发展规划（G200057001）
北京市自然科学基金（7062030）
北京市优秀人才基金（20051D0501820）
中国博士后科学基金（2005037379）
