摘要
目的:观察针刺预处理对全脑缺血大鼠海马CA1区c-fosmRNA及其蛋白表达的影响,并与缺血预处理的效果进行比较。方法:实验于2005-02/09在黑龙江中医药大学神经电生理实验室完成。①选用健康Wistar大鼠120只,雄性,体质量(250±20)g。采用随机数字表法将120只Wistar大鼠随机分为5组,每组24只:正常对照组:正常饲养,不干预。假手术组:暴露4条血管,不造模;脑缺血组:四动脉阻断法全脑缺血10min制作大鼠全脑缺血模型;脑缺血预处理组:预全脑缺血3min,再灌注24h后再次全脑缺血10min;针刺预处理组:术前7d给予针刺,双侧足三里、曲池穴,双侧连接全能脉冲电疗仪,频率为1Hz,电压为2V,30min/次,针刺百会30min/次,1次/d,7d后全脑缺血10min。②每组分别于术后12,24,48和72h麻醉状态下取材,每个时间点6只大鼠。免疫组织化学法检测海马CA1区c-Fos蛋白阳性细胞数,原位分子杂交技术检测大鼠海马CA1区c-fosmRNA表达。③计量资料差异比较采用单因素方差分析,两组间比较采用Newman-Keulsq检验。结果:120只大鼠均进入结果分析。①脑海马CA1区c-Fos阳性细胞数:正常对照组和假手术组无;脑缺血组少量表达,术后48h为高峰眼(23.35±7.68)个/mm2演,而脑缺血预处理组和针刺预处理组术后24,48,72h比较,差异不明显,但均明显高于脑缺血组(P<0.05)。②脑海马CA1区c-fosmRNA阳性细胞数:正常对照组和假手术组无;脑缺血组少量表达,术后72h为高峰眼(20.87±6.67)个/mm2,而脑缺血预处理组和针刺预处理组术后24,48,72h比较,差异不明显,但均明显高于脑缺血组(P<0.05)。结论:针刺预处理和脑缺血预处理均可能通过上调c-fosmRNA表达而减轻严重缺血后细胞凋亡,促成脑缺血耐受的产生。
AIM： To observe the effect of acupuncture precondition on c-fos mRNA and its protein expression in hippocampal CAI of the rats with global cerebral ischemic, in comparison with that of ischemic precondition.
METHODS： The experiment was conducted in the Electroneurophysiology Laboratory of Heilongjiang University of Traditional Chinese Medicine from February to September 2005. ①Totally 120 male Wistar rats with body mass （250±20） g were randomly divided into 5 groups with 24 rats in each group： Normal control group： routine fed without any intervention; shamoperation group： 4 vessels were exposed; cerebral ischemic group： 4-veSsel- blocked was conducted for 10 minutes to establish cerebral ischemia models; cerebral ischemic precondition group： 4 blood vessels were blocked for 3 minutes firstly, then 10 minutes after 24 hours of reperfusion; and acupuncture precondition group： electroacupuncture was given on zusanli, quchi and baihui, and the pulse electrotherapy was connected with the rats with frequency of 1 Hz, voltage 2 V, 30 minutes once, once daily, 7 days later, the rats were given 4-vessel-block for 10 minutes.②All rats were killed at the 12^th, 24^th, 48^th and 72^nd hours, respectively with 6 rats in each time point. Immunochistochemistry was applied to observe positive neurons of c-Fos protein in hippocampus CA1, and the in situ hybridization technique was used to observe c-fos mRNA expression in hippocampus CA1.③Single factor analysis of variance was adopted for comparison of difference of measurement data, and Newman- Keuls q-test for comparison between goups.
RESULTS： All 120 rats were involved in the result analysis. ①Positive neurons of c-Fos in hippocampus CAI： No neurons were found in the control and sham-operation groups; there were little expressions in the cerebral ischemic group, which reached the peak at the 48^th hour after operation [（23.35±7.68） cells/mm^2]; compared with the ischemic group, positive neurons of c-Fos protein expression in ischemic
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第19期125-127,共3页
Chinese Journal of Clinical Rehabilitation
