摘要
将人γ－干扰素（ＩＦＮ－γ）含信号肽ｃＤＮＡ构建入可表达逆转录病毒载体质粒ｐＬＸＳＮ， 体外包装成缺陷型逆转录病毒后分别感染人胃癌细胞株ＭＫＮ－４５及人肝癌细胞株ＨＨＣＬ，经筛选 后得到ＩＦＮ－γ基因修饰的肿瘤细胞，流式细胞仪分别检测了基因修饰肿瘤细胞的细胞周期及 ＨＬＡ－Ⅰ，Ⅱ类抗原表达；裸鼠皮下接种观察其致癌性是否变化，结果发现经ＩＦＮ－γ基因修饰后细 胞增殖减慢；ＨＬＡ－Ⅰ，Ⅱ类表达都有不同程度的增加，致肿瘤性也有一定程度的降低；为进一步研 究“肿瘤疫苗”提供了一定的依据。
The whole length cDNA of human interferon-γ(IFN-γ) gene including signal peptide was constructed to retroviral vector pLXSN and then packaged with PA317. A Human Hepetoma Cell Line(HHCL) and a human gastric cancer cell line (MKN-45) were infected by using supernatant containing retroviral vector with IFN-γ. the gene-modified tumor cell. were isolated by G418 selection. Some changes were found in cell cycle, expression of HLA class Ⅰ and classⅡ, and tumorigenecity. Elevated immunogenecity and abrogated rumerigenecity suggest a means for generating gene-modified tumor cell vaccine. for the treatment of cancer.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1996年第1期25-27,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
上海市科学技术发展基金
上海市高等学校科学技术发展基金
